PMID- 20676761
OWN - NLM
STAT- MEDLINE
DCOM- 20110105
LR  - 20211020
IS  - 1573-7446 (Electronic)
IS  - 0165-7380 (Print)
IS  - 0165-7380 (Linking)
VI  - 34
IP  - 7
DP  - 2010 Oct
TI  - N-acetylpenicillamine inhibits the replication of porcine reproductive and 
      respiratory syndrome virus in vitro.
PG  - 607-17
LID - 10.1007/s11259-010-9435-9 [doi]
AB  - Nitric oxide (NO) was proposed to be an important molecule against some 
      microorganisms. In this study, we investigated the inhibitory effect of NO on the 
      infection by porcine reproductive and respiratory syndrome virus (PRRSV) in vitro 
      and the role of NO in the defense against PRRSV. Our results indicated that 
      exogenous NO did not inhibit PRRSV infection. Unexpectedly, N-acetylpenicillamine 
      (NAP), a commonly used compound as negative control for NO-producing reagents, 
      inhibited PRRSV replication. Thus, the inhibition effect of NAP on PRRSV 
      replication was further explored. We found that the maximal inhibition effect of 
      NAP on PRRSV replication was achieved upon treatment 1 h after virus infection 
      and the virus yield was reduced by approximately 50 fold in the presence of 400 
      muM NAP. An obvious inhibitory effect on viral RNA and protein synthesis was also 
      observed. However, the inhibitory effect was only achieved at early phase of 
      virus infection. The normal virus yield could be restored upon the removal of NAP 
      treatment. The inhibitory effect might be caused by sulfhydryl-reducing capacity 
      and metal chelating properties of NAP. These studies suggested that (i) NO 
      production or NO synthase (NOS) expression profiling may not be a reliable index 
      for the immune response to PRRSV; (ii) NAP could inhibit the replication of 
      PRRSV.
FAU - Jiang, Yunbo
AU  - Jiang Y
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University, Wuhan, 430070, China. yunb_jiang@yahoo.com.cn
FAU - Fang, Liurong
AU  - Fang L
FAU - Luo, Rui
AU  - Luo R
FAU - Xiao, Shaobo
AU  - Xiao S
FAU - Chen, Huanchun
AU  - Chen H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100731
PL  - Switzerland
TA  - Vet Res Commun
JT  - Veterinary research communications
JID - 8100520
RN  - 0 (Antiviral Agents)
RN  - 0 (RNA, Viral)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 59-53-0 (N-acetylpenicillamine)
RN  - 79032-48-7 (S-Nitroso-N-Acetylpenicillamine)
RN  - GNN1DV99GX (Penicillamine)
SB  - IM
MH  - Animals
MH  - Antiviral Agents/*pharmacology
MH  - Cell Line
MH  - Dose-Response Relationship, Drug
MH  - Gene Expression Regulation, Viral/drug effects
MH  - Molecular Structure
MH  - Nitric Oxide
MH  - Penicillamine/*analogs & derivatives/pharmacology
MH  - Porcine respiratory and reproductive syndrome virus/*drug effects/physiology
MH  - RNA, Viral/metabolism
MH  - S-Nitroso-N-Acetylpenicillamine/pharmacology
MH  - Structure-Activity Relationship
MH  - Virus Replication/*drug effects/physiology
PMC - PMC7089432
EDAT- 2010/08/03 06:00
MHDA- 2011/01/06 06:00
PMCR- 2020/03/23
CRDT- 2010/08/03 06:00
PHST- 2010/07/21 00:00 [accepted]
PHST- 2010/08/03 06:00 [entrez]
PHST- 2010/08/03 06:00 [pubmed]
PHST- 2011/01/06 06:00 [medline]
PHST- 2020/03/23 00:00 [pmc-release]
AID - 9435 [pii]
AID - 10.1007/s11259-010-9435-9 [doi]
PST - ppublish
SO  - Vet Res Commun. 2010 Oct;34(7):607-17. doi: 10.1007/s11259-010-9435-9. Epub 2010 
      Jul 31.