PMID- 20676972
OWN - NLM
STAT- MEDLINE
DCOM- 20101202
LR  - 20100802
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 634
DP  - 2010
TI  - Mutagenesis protocols in Saccharomyces cerevisiae by in vivo overlap extension.
PG  - 3-14
LID - 10.1007/978-1-60761-652-8_1 [doi]
AB  - A high recombination frequency and its ease of manipulation has made 
      Saccharomyces cerevisiae a unique model eukaryotic organism to study homologous 
      recombination. Indeed, the well-developed recombination machinery in S. 
      cerevisiae facilitates the construction of mutant libraries for directed 
      evolution experiments. In this context, in vivo overlap extension (IVOE) is a 
      particularly attractive protocol that takes advantage of the eukaryotic apparatus 
      to carry out combinatorial saturation mutagenesis, site-directed recombination or 
      site-directed mutagenesis, avoiding ligation steps and additional PCR reactions 
      that are common to standard in vitro protocols.
FAU - Alcalde, Miguel
AU  - Alcalde M
AD  - Instituto de Catalisis y Petroleoquimica (CSIC), Madrid, Spain. 
      malcalde@icp.csic.es
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Culture Media)
SB  - IM
MH  - Culture Media
MH  - Directed Molecular Evolution
MH  - *Mutagenesis
MH  - Recombination, Genetic
MH  - Saccharomyces cerevisiae/*genetics
EDAT- 2010/08/03 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/08/03 06:00
PHST- 2010/08/03 06:00 [entrez]
PHST- 2010/08/03 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - 10.1007/978-1-60761-652-8_1 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;634:3-14. doi: 10.1007/978-1-60761-652-8_1.