PMID- 20677157
OWN - NLM
STAT- MEDLINE
DCOM- 20101210
LR  - 20100802
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 27
IP  - 4
DP  - 2010 Aug
TI  - [Application of fluorescence in situ hybridization to prenatal diagnosis of 
      aneuploidy in 110 uncultured amniotic fluid samples].
PG  - 453-6
LID - 10.3760/cma.j.issn.1003-9406.2010.04.021 [doi]
AB  - OBJECTIVE: To optimize the prenatal diagnosis platform by using domestically made 
      fluorescence in situ hybridization(FISH) kit and to explore the clinical 
      application of FISH to rapid prenatal diagnosis of a wide range of chromosomal 
      abnormalities. METHODS: Amniotic fluid samples from 110 pregnant women were 
      studied with the rapid prenatal diagnosis method of FISH and the conventional 
      cell culture method of karyotyping, the results from both methods were compared. 
      RESULTS: Four cases of trisomy 21, 1 case of trisomy 18, 58 cases of 46, XX, and 
      47 cases of 46, XY were detected by FISH in the 110 amniotic fluid samples. It is 
      concordant with the results from conventional karyotype analysis. The concordance 
      rate is 100%. CONCLUSION: Domestically made FISH kit can be used to rapidly and 
      accurately detect the most common chromosome aneuploidies by using less sample 
      volume while the price is relatively low. FISH can be a reliable and rapid 
      prenatal diagnostic tool as an adjunct to classical cytogenetic study. It can be 
      used for rapid and accurate prenatal diagnosis of women with high risk of 
      maternal serum screening.
FAU - Liu, Han
AU  - Liu H
AD  - Birth Health Perinatal Medicine Institute, Women and Children Center of 
      Guangzhou, Guangzhou, Guangdong, 510180 PR China.
FAU - Liao, Can
AU  - Liao C
FAU - Huang, Yi-ning
AU  - Huang YN
FAU - Pan, Min
AU  - Pan M
FAU - Yi, Cui-xing
AU  - Yi CX
FAU - Yuan, Si-min
AU  - Yuan SM
FAU - Hu, Shun-yan
AU  - Hu SY
FAU - Zhong, Hu-zhu
AU  - Zhong HZ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - Adult
MH  - Amniocentesis
MH  - Amniotic Fluid
MH  - *Aneuploidy
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 18/*genetics
MH  - Down Syndrome/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping/methods
MH  - Nucleic Acid Hybridization
MH  - Pregnancy
MH  - Prenatal Diagnosis/*methods
MH  - Trisomy
EDAT- 2010/08/03 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/08/03 06:00
PHST- 2010/08/03 06:00 [entrez]
PHST- 2010/08/03 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - 940627067 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2010.04.021 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2010 Aug;27(4):453-6. doi: 
      10.3760/cma.j.issn.1003-9406.2010.04.021.