PMID- 20678654
OWN - NLM
STAT- MEDLINE
DCOM- 20101215
LR  - 20131121
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 82
IP  - 3
DP  - 2010 Aug 15
TI  - Simultaneous quantification of amphetamines, caffeine and ketamine in urine by 
      hollow fiber liquid phase microextraction combined with gas chromatography-flame 
      ionization detector.
PG  - 969-75
LID - 10.1016/j.talanta.2010.06.001 [doi]
AB  - A method of hollow fiber (HF) liquid phase microextraction (LPME) combined with 
      gas chromatography (GC)-flame ionization detection (FID) was developed for the 
      simultaneous quantification of trace amphetamine (AP), methamphetamine (MA), 
      methylenedioxyamphetamine (MDA), methylenedioxymethamphetamine (MDMA), caffeine 
      and ketamine (KT) in drug abuser urine samples. The factors affecting on the 
      extraction of six target analytes by HF-LPME were investigated and optimized, and 
      the subsequent analytical performance evaluation and real sample analysis were 
      performed by the extraction of six target analytes in sample solution containing 
      30% NaCl (pH 12.5) for 20 min with extraction temperature of 30 degrees C and 
      stirring rate of 1000 rpm. Under such optimal conditions, the limits of detection 
      (LODs, S/N=3) for the six target analytes were ranged from 8 microg/L (AP, KT) to 
      82 microg/L (MDA), with the enrichment factors (EFs) of 5-227 folds, and the 
      relative standard deviations (RSDs, n=7) were in the range of 6.9-14.1%. The 
      correlation coefficients of the calibration for the six target analytes over the 
      dynamic linear range were higher than 0.9958. The application feasibility of 
      HF-LPME-GC-FID in illegal drug monitoring was demonstrated by analyzing drug 
      abuser urine samples, and the recoveries of target drugs for the spiked sample 
      ranging from 75.2% to 119.3% indicated an excellent anti-interference capability 
      of the developed method. The proposed method is simple, effective, sensitive and 
      low-cost, and provides a much more accurate and sensitive detection platform over 
      the conventional analytical techniques (such as immunological assay) for drug 
      abuse analysis.
CI  - Copyright 2010 Elsevier B.V. All rights reserved.
FAU - Xiong, Jun
AU  - Xiong J
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
FAU - Chen, Jie
AU  - Chen J
FAU - He, Man
AU  - He M
FAU - Hu, Bin
AU  - Hu B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100608
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Amphetamines)
RN  - 3G6A5W338E (Caffeine)
RN  - 44RAL3456C (Methamphetamine)
RN  - 690G0D6V8H (Ketamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Amphetamines/*urine
MH  - Caffeine/*urine
MH  - Calibration
MH  - Chromatography, Gas/*methods
MH  - Drug Monitoring/methods
MH  - Flame Ionization
MH  - Humans
MH  - Ketamine/*urine
MH  - Limit of Detection
MH  - Methamphetamine/urine
MH  - N-Methyl-3,4-methylenedioxyamphetamine/urine
MH  - Reproducibility of Results
MH  - Substance-Related Disorders/*diagnosis
EDAT- 2010/08/04 06:00
MHDA- 2010/12/16 06:00
CRDT- 2010/08/04 06:00
PHST- 2010/01/19 00:00 [received]
PHST- 2010/05/31 00:00 [revised]
PHST- 2010/06/01 00:00 [accepted]
PHST- 2010/08/04 06:00 [entrez]
PHST- 2010/08/04 06:00 [pubmed]
PHST- 2010/12/16 06:00 [medline]
AID - S0039-9140(10)00427-3 [pii]
AID - 10.1016/j.talanta.2010.06.001 [doi]
PST - ppublish
SO  - Talanta. 2010 Aug 15;82(3):969-75. doi: 10.1016/j.talanta.2010.06.001. Epub 2010 
      Jun 8.