PMID- 20678921
OWN - NLM
STAT- MEDLINE
DCOM- 20110207
LR  - 20171116
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 26
IP  - 2
DP  - 2010 Oct 15
TI  - Molecularly imprinted polymeric microspheres for determination of bovine serum 
      albumin based on flow injection chemiluminescence sensor.
PG  - 632-7
LID - 10.1016/j.bios.2010.07.009 [doi]
AB  - A novel flow injection chemiluminescence (FI-CL) sensor for the determination of 
      bovine serum albumin (BSA) using molecularly imprinted polymeric microspheres 
      (MIPMs) as recognition element is reported. The BSA-MIPM was synthesized by 
      suspension polymerization in toluene, using methacrylic acid (MAA) as functional 
      monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker in the bovine 
      serum albumin template molecule. Scanning electron microscope (SEM) was employed 
      to characterize the surface morphology of the resultant imprinted microspheres. 
      Molecular modeling was employed to simulate the possible recognition process of 
      the MIPM. Then the synthesized BSA-MIPM was employed as recognition element by 
      packing into flow cell to establish a novel FI-CL sensor. The chemiluminescence 
      (CL) intensity was correlated linear with the concentration of BSA over the range 
      of 1.0x10(-8) to 5.0x10(-6) g mL(-1) and the detection limit was 1.5x10(-9) g 
      mL(-1). The relative standard deviation (RSD) for the determination of 1.0x10(-7) 
      g mL(-1) BSA was 1.4% (n=11). The sensor is reusable and has a great improvement 
      in sensitivity and selectivity for CL analysis. As a result, the new MIPM-CL 
      sensor had been successfully applied to the determination of BSA in milk samples.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Yu, Jinghua
AU  - Yu J
AD  - Shandong Provincial Key Laboratory of Fluorine Chemistry and Chemical Materials, 
      School of Chemistry and Chemical Engineering, University of Jinan, No. 106, Jiwei 
      Road, Jinan 250022, China.
FAU - Wan, Fuwei
AU  - Wan F
FAU - Zhang, Congcong
AU  - Zhang C
FAU - Yan, Mei
AU  - Yan M
FAU - Zhang, Xiaona
AU  - Zhang X
FAU - Wang, Shaowei
AU  - Wang S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100713
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Polymers)
RN  - 27432CM55Q (Serum Albumin, Bovine)
SB  - IM
MH  - Biosensing Techniques/*instrumentation
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Flow Injection Analysis/*instrumentation
MH  - Luminescent Measurements/*instrumentation
MH  - Microspheres
MH  - Polymers/*chemistry
MH  - Serum Albumin, Bovine/*analysis
MH  - Surface Properties
EDAT- 2010/08/04 06:00
MHDA- 2011/02/08 06:00
CRDT- 2010/08/04 06:00
PHST- 2010/02/08 00:00 [received]
PHST- 2010/06/07 00:00 [revised]
PHST- 2010/07/05 00:00 [accepted]
PHST- 2010/08/04 06:00 [entrez]
PHST- 2010/08/04 06:00 [pubmed]
PHST- 2011/02/08 06:00 [medline]
AID - S0956-5663(10)00384-2 [pii]
AID - 10.1016/j.bios.2010.07.009 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2010 Oct 15;26(2):632-7. doi: 10.1016/j.bios.2010.07.009. 
      Epub 2010 Jul 13.