PMID- 20679961
OWN - NLM
STAT- MEDLINE
DCOM- 20110314
LR  - 20100916
IS  - 1744-6880 (Electronic)
IS  - 1744-6872 (Linking)
VI  - 20
IP  - 10
DP  - 2010 Oct
TI  - Development of new HLA-B*3505 genotyping method using Invader assay.
PG  - 630-3
LID - 10.1097/FPC.0b013e32833ddc0a [doi]
AB  - Several pharmacogenetic studies have revealed strong associations between 
      specific human leukocyte antigen (HLA) alleles and the susceptibility to drug 
      hypersensitivity. Recently, we reported HLA-B*3505 as a strong genetic biomarker 
      for the nevirapine-induced skin rash in Thai population. Here, we developed a new 
      HLA-B*3505 genotyping method by a combination of the Universal Invader assay and 
      sequence-specific primer PCR. From the sequence alignment of 68 HLA-B alleles in 
      the Thai population, we selected the two most discriminative SNPs (rs1140412 and 
      rs4997052) as target SNP sites. When we carried out the assay using 324 Thai 
      individuals, fluorescence intensities of HLA-B*3505-positive and 
      HLA-B*3505-negative samples were apparently discriminated at the endpoint of the 
      reaction. Our results were 100% concordant with those obtained by a 
      sequence-based typing method. As our assay is simple and rapid, we believe our 
      method will be a useful tool for pharmacogenetic testing of the 
      nevirapine-induced skin rash.
FAU - Hosono, Naoya
AU  - Hosono N
AD  - Laboratory for Genotyping Development, University of Tokyo, Japan. 
      nhosono@src.riken.jp
FAU - Chantarangsu, Soranun
AU  - Chantarangsu S
FAU - Kiyotani, Kazuma
AU  - Kiyotani K
FAU - Takata, Sadaaki
AU  - Takata S
FAU - Tsuchiya, Yumiko
AU  - Tsuchiya Y
FAU - Mahasirimongkol, Surakameth
AU  - Mahasirimongkol S
FAU - Chantratita, Wasun
AU  - Chantratita W
FAU - Mushiroda, Taisei
AU  - Mushiroda T
FAU - Nakamura, Yusuke
AU  - Nakamura Y
FAU - Kubo, Michiaki
AU  - Kubo M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pharmacogenet Genomics
JT  - Pharmacogenetics and genomics
JID - 101231005
RN  - 0 (HLA-B Antigens)
SB  - IM
MH  - Base Sequence
MH  - Biological Assay/*methods
MH  - Fluorescence
MH  - Genotype
MH  - HLA-B Antigens/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction/*methods
MH  - Sequence Analysis, DNA/*methods
EDAT- 2010/08/04 06:00
MHDA- 2011/03/15 06:00
CRDT- 2010/08/04 06:00
PHST- 2010/08/04 06:00 [entrez]
PHST- 2010/08/04 06:00 [pubmed]
PHST- 2011/03/15 06:00 [medline]
AID - 10.1097/FPC.0b013e32833ddc0a [doi]
PST - ppublish
SO  - Pharmacogenet Genomics. 2010 Oct;20(10):630-3. doi: 10.1097/FPC.0b013e32833ddc0a.