PMID- 20680585
OWN - NLM
STAT- MEDLINE
DCOM- 20101206
LR  - 20211020
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 656
DP  - 2010
TI  - Lipid detection, identification, and imaging single cells with SIMS.
PG  - 85-97
LID - 10.1007/978-1-60761-746-4_4 [doi]
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) can be utilized to map 
      the distribution of various molecules on a surface with submicrometer resolution. 
      Many of its biological applications have been in the study of membrane lipids, 
      such as phospholipids and cholesterol. For these studies, the effectiveness of 
      chemical mapping is limited by low signal intensity from various biomolecules. 
      Because of the high-energy nature of the SIMS ionization process, many molecules 
      are identified by detection of characteristic fragments. Cluster ion sources are 
      able to increase ionization, leading to increased information collected from a 
      surface. In this chapter, we highlight the utility of SIMS to image lipids at 
      single cells. Particularly, we will describe sample preparation, data collection, 
      and the analysis of lipids for two systems; rat oligodendrocytes and Tetrahymena 
      thermophila. SIMS spectra yield information regarding lipid identity and 
      concentration across cell surface.
FAU - Heien, Michael L
AU  - Heien ML
AD  - Department of Chemistry, The University of Arizona, Tucson, AZ, USA.
FAU - Piehowski, Paul D
AU  - Piehowski PD
FAU - Winograd, Nicholas
AU  - Winograd N
FAU - Ewing, Andrew G
AU  - Ewing AG
LA  - eng
GR  - R01 EB002016/EB/NIBIB NIH HHS/United States
GR  - R01EB002016/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Lipids)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Lipids/*chemistry
MH  - Oligodendroglia/cytology
MH  - Rats
MH  - Spectrometry, Mass, Secondary Ion/*methods
MH  - Tetrahymena thermophila/cytology
EDAT- 2010/08/04 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/08/04 06:00
PHST- 2010/08/04 06:00 [entrez]
PHST- 2010/08/04 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - 10.1007/978-1-60761-746-4_4 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;656:85-97. doi: 10.1007/978-1-60761-746-4_4.