PMID- 20680592
OWN - NLM
STAT- MEDLINE
DCOM- 20101206
LR  - 20141120
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 656
DP  - 2010
TI  - Cellular imaging using matrix-enhanced and metal-assisted SIMS.
PG  - 197-208
LID - 10.1007/978-1-60761-746-4_11 [doi]
AB  - Imaging mass spectrometry (IMS) allows the direct investigation of both the 
      identity and the spatial distribution of the entire molecular content directly in 
      tissue sections, single cells, and many other biological surfaces. We describe 
      here the steps required to retrieve the molecular information from tissue 
      sections using matrix-enhanced (ME) and metal-assisted (MetA) secondary ion mass 
      spectrometry (SIMS). Surface metallization by plasma coating enhances 
      desorption/ionization of membrane components such as lipids and sterols in 
      imaging time-of-flight (ToF) SIMS of tissues and cells. High-resolution images of 
      cholesterol and other membrane components can be obtained for single 
      neuroblastoma cells and reveal subcellular details. Alternatively, in ME-SIMS, 
      2,5-dihydroxybenzoic acid electrosprayed on neuroblastoma cells allows intact 
      molecular ion imaging of phosphatidylcholine (PC) and sphingomyelin (SM) at the 
      cellular level.
FAU - Altelaar, A F Maarten
AU  - Altelaar AF
AD  - Biomolecular Mass Spectrometry and Proteomics Group, Utrecht University, Utrecht, 
      The Netherlands.
FAU - Piersma, Sander R
AU  - Piersma SR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Cells/*cytology/metabolism
MH  - Cells, Cultured
MH  - Diagnostic Imaging/*methods
MH  - In Vitro Techniques
MH  - Male
MH  - Neuroblastoma/pathology
MH  - Rats
MH  - Snails/cytology
MH  - Spectrometry, Mass, Secondary Ion/*methods
EDAT- 2010/08/04 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/08/04 06:00
PHST- 2010/08/04 06:00 [entrez]
PHST- 2010/08/04 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - 10.1007/978-1-60761-746-4_11 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;656:197-208. doi: 10.1007/978-1-60761-746-4_11.