PMID- 20680597
OWN - NLM
STAT- MEDLINE
DCOM- 20101206
LR  - 20100803
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 656
DP  - 2010
TI  - Applying imaging ToF-SIMS and PCA in differentiation of tissue types.
PG  - 267-81
LID - 10.1007/978-1-60761-746-4_16 [doi]
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) has proven to be an 
      extremely powerful tool for characterizing chemical distributions within 
      biological cells and tissues. However, differentiating biological samples, e.g., 
      cancerous cells from their normal counterparts or benign tissues from malignant 
      tissues, presents unique challenges to ToF-SIMS. Repeatable differentiation of 
      such samples, especially formalin-fixed paraffin-embedded (FFPE) histological 
      specimens, could be used to improve tissue-based diagnosis and aid in prognosis 
      decisions. In this chapter, we describe a strategy for characterizing and 
      differentiating FFPE tissues. ToF-SIMS was used to image deparaffinized FFPE 
      mouse embryos and differentiate tissue types. The robustness and repeatability of 
      the method was determined by analyzing ten tissue slices from three different 
      embryos over a period of 1 month. Using principal component analysis (PCA) to 
      reduce the spectral data generated by ToF-SIMS, histopathologically identified 
      tissue types of the mouse embryos can be differentiated based on the 
      characteristic differences in their mass spectra.
FAU - Wu, Ligang
AU  - Wu L
AD  - Seagate Technology US, Fremont, CA, USA.
FAU - Felton, James S
AU  - Felton JS
FAU - Wu, Kuang Jen J
AU  - Wu KJ
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Cell Differentiation/physiology
MH  - Diagnostic Imaging/*methods
MH  - Embryo, Mammalian/cytology
MH  - Female
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Principal Component Analysis
MH  - Spectrometry, Mass, Secondary Ion/*methods
EDAT- 2010/08/04 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/08/04 06:00
PHST- 2010/08/04 06:00 [entrez]
PHST- 2010/08/04 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - 10.1007/978-1-60761-746-4_16 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;656:267-81. doi: 10.1007/978-1-60761-746-4_16.