PMID- 20681807
OWN - NLM
STAT- MEDLINE
DCOM- 20110224
LR  - 20101102
IS  - 1943-3670 (Electronic)
IS  - 0022-3492 (Linking)
VI  - 81
IP  - 11
DP  - 2010 Nov
TI  - Bone morphogenetic protein-7 enhances cementoblast function in vitro.
PG  - 1663-74
LID - 10.1902/jop.2010.100074 [doi]
AB  - BACKGROUND: Bone morphogenetic protein (BMP)-7 is a potent bone-inducing factor 
      and was shown to promote periodontal regeneration in vivo and in vitro; however, 
      to our knowledge, the specific effect of BMP-7 on cementoblasts has not been 
      defined. We aimed to investigate the effects of BMP-7 on cementoblasts, which are 
      cells responsible for tooth root-cementum formation. We hypothesized that BMP-7 
      would regulate mineralized tissue-associated genes in cementoblasts and influence 
      the expression profile of genes associated with cementoblast extracellular matrix 
      (ECM) and cell adhesion molecules (CAMs). METHODS: A murine immortalized 
      cementoblast cell line (OCCM.30) was cultured with and without 50 ng/ml BMP-7. 
      After 72 hours, total RNA was isolated, and mRNA levels for bone/cementum 
      markers, including bone sialoprotein (BSP), osteocalcin (OCN), osteopontin (OPN), 
      and runt-related transcription factor-2 (Runx2), were investigated by real-time 
      quantitative reverse transcription-polymerase chain reaction (Q-PCR). In vitro 
      mineral nodule formation was assayed on day 8 using von Kossa staining. A 
      pathway-specific gene-expression array was used to determine BMP-7-responsive ECM 
      and CAM genes in cementoblasts. RESULTS: Mineralized tissue markers were strongly 
      regulated by BMP-7, with an almost three-fold increase in BSP and OCN transcripts 
      and significant increases in OPN and Runx2 mRNA expressions. BMP-7 treatment 
      markedly stimulated cementoblast-mediated biomineralization in vitro compared to 
      untreated cells at day 8. BMP-7 treatment altered the OCCM.30 expression profile 
      for ECM and CAM functional gene groups. BMP-7 tended to increase the expression 
      of collagens and matrix metalloproteinases (MMPs), mildly decreased tissue 
      inhibitors of MMPs (TIMPs), and had mixed regulatory effects on integrins. Using 
      Q-PCR, selected array results were confirmed, including a significant 
      BMP-7-induced increase in MMP-3 and a decrease in TIMP-2 mRNA expression. 
      CONCLUSION: These results support the promising applications of BMP-7 in 
      therapies aimed at regenerating periodontal tissues lost as a consequence of 
      disease.
FAU - Hakki, Sema S
AU  - Hakki SS
AD  - Department of Periodontology, Faculty of Dentistry, Selcuk University, Konya, 
      Turkey. sshakki@yahoo.com
FAU - Foster, Brian L
AU  - Foster BL
FAU - Nagatomo, Kanako J
AU  - Nagatomo KJ
FAU - Bozkurt, S Buket
AU  - Bozkurt SB
FAU - Hakki, Erdogan E
AU  - Hakki EE
FAU - Somerman, Martha J
AU  - Somerman MJ
FAU - Nohutcu, Rahime M
AU  - Nohutcu RM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100803
PL  - United States
TA  - J Periodontol
JT  - Journal of periodontology
JID - 8000345
RN  - 0 (Bone Morphogenetic Protein 7)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Integrins)
RN  - 0 (Runx2 protein, mouse)
RN  - 0 (Spp1 protein, mouse)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - 0 (bmp7 protein, mouse)
RN  - 104982-03-8 (Osteocalcin)
RN  - 106441-73-0 (Osteopontin)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.17 (Mmp3 protein, mouse)
SB  - IM
MH  - Animals
MH  - Bone Morphogenetic Protein 7/*pharmacology
MH  - Calcification, Physiologic/drug effects
MH  - Cell Adhesion Molecules/drug effects
MH  - Cell Differentiation/drug effects
MH  - Cell Line
MH  - Collagen/drug effects
MH  - Core Binding Factor Alpha 1 Subunit/drug effects
MH  - Dental Cementum/cytology/*drug effects
MH  - Extracellular Matrix Proteins/drug effects
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/drug effects
MH  - Integrins/drug effects
MH  - Matrix Metalloproteinase 3/drug effects
MH  - Matrix Metalloproteinases/drug effects
MH  - Mice
MH  - Osteocalcin/drug effects
MH  - Osteopontin/drug effects
MH  - Protein Array Analysis
MH  - Time Factors
MH  - Tissue Inhibitor of Metalloproteinase-2/drug effects
MH  - Tissue Inhibitor of Metalloproteinases/drug effects
MH  - Tooth Root/cytology/drug effects
MH  - Up-Regulation
EDAT- 2010/08/05 06:00
MHDA- 2011/02/25 06:00
CRDT- 2010/08/05 06:00
PHST- 2010/08/05 06:00 [entrez]
PHST- 2010/08/05 06:00 [pubmed]
PHST- 2011/02/25 06:00 [medline]
AID - 10.1902/jop.2010.100074 [doi]
PST - ppublish
SO  - J Periodontol. 2010 Nov;81(11):1663-74. doi: 10.1902/jop.2010.100074. Epub 2010 
      Aug 3.