PMID- 20682885
OWN - NLM
STAT- MEDLINE
DCOM- 20100908
LR  - 20211020
IS  - 1476-1645 (Electronic)
IS  - 0002-9637 (Print)
IS  - 0002-9637 (Linking)
VI  - 83
IP  - 2
DP  - 2010 Aug
TI  - Diagnostic accuracy and feasibility of serological tests on filter paper samples 
      for outbreak detection of T.b. gambiense human African trypanosomiasis.
PG  - 374-9
LID - 10.4269/ajtmh.2010.09-0735 [doi]
AB  - Control of human African trypanosomiasis (HAT) in the Democratic Republic of 
      Congo is based on mass population screening by mobile teams; a costly and 
      labor-intensive approach. We hypothesized that blood samples collected on filter 
      paper by village health workers and processed in a central laboratory might be a 
      cost-effective alternative. We estimated sensitivity and specificity of 
      micro-card agglutination test for trypanosomiasis (micro-CATT) and enzyme-linked 
      immunosorbent assay (ELISA)/T.b. gambiense on filter paper samples compared with 
      parasitology-based case classification and used the results in a Monte Carlo 
      simulation of a lot quality assurance sampling (LQAS) approach. Micro-CATT and 
      ELISA/T.b. gambiense showed acceptable sensitivity (92.7% [95% CI 87.4-98.0%] and 
      82.2% [95% CI 75.3-90.4%]) and very high specificity (99.4% [95% CI 99.0-99.9%] 
      and 99.8% [95% CI 99.5-100%]), respectively. Conditional on high sample size per 
      lot (> or = 60%), both tests could reliably distinguish a 2% from a zero 
      prevalence at village level. Alternatively, these tests could be used to identify 
      individual HAT suspects for subsequent confirmation.
FAU - Hasker, Epco
AU  - Hasker E
AD  - Institute of Tropical Medicine Antwerp, Belgium. ehasker@itg.be
FAU - Lutumba, Pascal
AU  - Lutumba P
FAU - Mumba, Dieudonne
AU  - Mumba D
FAU - Lejon, Veerle
AU  - Lejon V
FAU - Buscher, Phillipe
AU  - Buscher P
FAU - Kande, Victor
AU  - Kande V
FAU - Muyembe, Jean Jacques
AU  - Muyembe JJ
FAU - Menten, Joris
AU  - Menten J
FAU - Robays, Jo
AU  - Robays J
FAU - Boelaert, Marleen
AU  - Boelaert M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Trop Med Hyg
JT  - The American journal of tropical medicine and hygiene
JID - 0370507
RN  - 0 (Antibodies, Protozoan)
SB  - IM
MH  - Agglutination Tests/*standards
MH  - Antibodies, Protozoan/blood
MH  - Blood Specimen Collection/instrumentation/methods
MH  - Democratic Republic of the Congo/epidemiology
MH  - *Disease Outbreaks
MH  - Enzyme-Linked Immunosorbent Assay/*standards
MH  - Filtration/instrumentation
MH  - Humans
MH  - Latex Fixation Tests/standards
MH  - Monte Carlo Method
MH  - Paper
MH  - ROC Curve
MH  - Sensitivity and Specificity
MH  - Specimen Handling
MH  - Trypanosoma brucei gambiense/*immunology
MH  - Trypanosomiasis, African/*diagnosis/epidemiology
PMC - PMC2911188
EDAT- 2010/08/05 06:00
MHDA- 2010/09/09 06:00
PMCR- 2011/08/05
CRDT- 2010/08/05 06:00
PHST- 2010/08/05 06:00 [entrez]
PHST- 2010/08/05 06:00 [pubmed]
PHST- 2010/09/09 06:00 [medline]
PHST- 2011/08/05 00:00 [pmc-release]
AID - 83/2/374 [pii]
AID - 10.4269/ajtmh.2010.09-0735 [doi]
PST - ppublish
SO  - Am J Trop Med Hyg. 2010 Aug;83(2):374-9. doi: 10.4269/ajtmh.2010.09-0735.