PMID- 20683100
OWN - NLM
STAT- MEDLINE
DCOM- 20101216
LR  - 20100804
IS  - 1735-1502 (Print)
IS  - 1735-1502 (Linking)
VI  - 9
IP  - 2
DP  - 2010 Jun
TI  - Cytotoxicity of human cord blood natural killer cells is enhanced by recombinant 
      interleukin-15.
PG  - 69-77
AB  - Hematopoietic cord blood (CB) stem cell transplantation has more advantages to 
      other cell sources because of lower Graft Versus Host Disease (GVHD). 
      Interleukin-15(IL-15) is an immunoregulatory cytokine, known to enhance cytolytic 
      function of cord Natural Killer (NK) cells. The aim of this study was to 
      investigate the effect of IL-15 on NK cytotoxicity simultaneously in different 
      cell death stages. We compared the ability of IL-15 to enhance the NK 
      cytotoxicity of CB in comparison to adult blood Mononuclear Cells (MNCs) against 
      K562 target cells by co-staining with AnnexinV-FITC and Propidium Iodide after 
      3.5 h incubation at 37  degrees C and 5% CO(2) by using flow cytometric method. We also 
      evaluated phenotypic changes after treatment by IL-15 in both cell sources. Our 
      results indicated that CB samples had lower level of apoptosis, while necrosis 
      was negligible; also by escalating Effector: Target (E: T), we got higher level 
      of apoptosis and necrosis in peripheral blood (PB). NK activity of cord and adult 
      MNCs was enhanced by incubation with IL-15 (10 ng/ml) for 72 h with significantly 
      higher results of PB in comparison to CB (p<0.0001). Moreover, IL-15 increased 
      the percentage of CD3-/CD56+ and CD25+ cells after 72 h incubation. Results 
      showed incubation with human recombinant (hr) IL-15 for 3 days increased NK 
      activity. Taken together, these results indicated that NK cytotoxicity of CB MNCs 
      could be augmented by human recombinant (hr) IL-15, but this activity did not 
      reach to same level of PB counterparts. We established that CD25 expression on CB 
      MNCs could be increased with IL-15, in 72-hour cultures, but to a lesser degree 
      compared to that on corresponding adult PB MNCs.
FAU - Saghafi, Shiva
AU  - Saghafi S
AD  - Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares 
      University, Tehran, Iran.
FAU - Pourfathollah, Ali Akbar
AU  - Pourfathollah AA
FAU - Kheirandish, Maryam
AU  - Kheirandish M
FAU - Azimdoust, Anahita
AU  - Azimdoust A
FAU - Behnia, Massoud
AU  - Behnia M
FAU - Shahjahani, Mohammad
AU  - Shahjahani M
FAU - Moin, Mostafa
AU  - Moin M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Iran
TA  - Iran J Allergy Asthma Immunol
JT  - Iranian journal of allergy, asthma, and immunology
JID - 101146178
RN  - 0 (Interleukin-15)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Apoptosis/drug effects
MH  - Cells, Cultured
MH  - Cytotoxicity, Immunologic/*drug effects
MH  - Fetal Blood/*cytology
MH  - Humans
MH  - Immunophenotyping
MH  - Interleukin-15/*pharmacology
MH  - Killer Cells, Natural/*drug effects/immunology
MH  - Necrosis
MH  - Recombinant Proteins/pharmacology
EDAT- 2010/08/05 06:00
MHDA- 2010/12/17 06:00
CRDT- 2010/08/05 06:00
PHST- 2010/08/05 06:00 [entrez]
PHST- 2010/08/05 06:00 [pubmed]
PHST- 2010/12/17 06:00 [medline]
AID - 09026977 [pii]
PST - ppublish
SO  - Iran J Allergy Asthma Immunol. 2010 Jun;9(2):69-77.