PMID- 20683395
OWN - NLM
STAT- MEDLINE
DCOM- 20101015
LR  - 20221207
IS  - 1525-1438 (Electronic)
IS  - 1048-891X (Linking)
VI  - 20
IP  - 6
DP  - 2010 Aug
TI  - Human telomerase RNA gene amplification detection increases the specificity of 
      cervical intraepithelial neoplasia screening.
PG  - 912-7
LID - 10.1111/IGC.0b013e3181e5c424 [doi]
AB  - OBJECTIVES: The purpose of this study was to evaluate the clinical significance 
      of genomic amplification of the human telomerase RNA gene (TERC) for cervical 
      cancer screening and explore whether it can serve as a biomarker to improve the 
      specificity of human papillomavirus (HPV) DNA testing for cervical cancer 
      screening. METHODS: One hundred twenty women, including 20 cases of normal 
      (control), 14 cases of cervical intraepithelial neoplasia grade I (CIN I), 35 
      cases of CIN II, 36 cases of CIN III, and 15 cases of squamous cervical cancer 
      diagnosed by histopathologic evaluation, were subjected to cytopathologic 
      examination, TERC detection by fluorescence in situ hybridization (FISH), and HPV 
      DNA testing by Hybrid Capture II. RESULTS: TERC amplification was significantly 
      associated with cytopathologic diagnosis (P < 0.001) and histopathologic 
      evaluation (P < 0.001). The positive rate of TERC gain was significantly higher 
      in patients with CIN III or squamous cervical cancer than in patients with CIN I 
      or those in the control group (P < 0.001). The specificity and positive 
      predictive value of FISH for detecting CIN II or more severe cervical lesions (> 
      or = CIN II) were obviously higher than those of HPV DNA testing (97.1% vs 52.9%, 
      98.7% vs 83.8%). CONCLUSIONS: TERC amplification analyzed by FISH may serve as an 
      adjunctive test to HPV DNA testing for improving the specificity and positive 
      predictive value of cervical cancer screening.
FAU - Zheng, Lei
AU  - Zheng L
AD  - Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, 
      Guangzhou, Guangdong, China.
FAU - Liu, Ai-Lan
AU  - Liu AL
FAU - Qi, Tao
AU  - Qi T
FAU - Wang, Qian
AU  - Wang Q
FAU - Cai, Zhen
AU  - Cai Z
FAU - Su, Ya-Juan
AU  - Su YJ
FAU - Hu, Yan-Wei
AU  - Hu YW
FAU - Liu, Guo-Bing
AU  - Liu GB
FAU - Wei, Li-Hui
AU  - Wei LH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Gynecol Cancer
JT  - International journal of gynecological cancer : official journal of the 
      International Gynecological Cancer Society
JID - 9111626
RN  - 0 (telomerase RNA)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/diagnosis/genetics
MH  - Chi-Square Distribution
MH  - Cohort Studies
MH  - Diagnosis, Differential
MH  - *Early Detection of Cancer
MH  - Female
MH  - *Gene Amplification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Mass Screening/methods
MH  - Middle Aged
MH  - RNA/*genetics
MH  - Sensitivity and Specificity
MH  - Statistics, Nonparametric
MH  - Telomerase/*genetics
MH  - Uterine Cervical Dysplasia/diagnosis/genetics
MH  - Uterine Cervical Neoplasms/*diagnosis/*genetics
EDAT- 2010/08/05 06:00
MHDA- 2010/10/16 06:00
CRDT- 2010/08/05 06:00
PHST- 2010/08/05 06:00 [entrez]
PHST- 2010/08/05 06:00 [pubmed]
PHST- 2010/10/16 06:00 [medline]
AID - 00009577-201008000-00002 [pii]
AID - 10.1111/IGC.0b013e3181e5c424 [doi]
PST - ppublish
SO  - Int J Gynecol Cancer. 2010 Aug;20(6):912-7. doi: 10.1111/IGC.0b013e3181e5c424.