PMID- 20686238
OWN - NLM
STAT- MEDLINE
DCOM- 20101214
LR  - 20190720
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 33
IP  - 8
DP  - 2010
TI  - Differential expression of ezrin and CLP36 in the two layers of 
      syncytiotrophoblast in rats.
PG  - 1400-6
AB  - The syncytiotrophoblast, which regulates maternal-fetal transfer of drugs, 
      consists of a single layer in humans, but two layers, i.e., SynI and SynII, in 
      rodents. Polar distribution of transporters in the apical and basal plasma 
      membranes of syncytiotrophoblast is important for placental function in terms of 
      vectorial transport of substrates, but the mechanisms that control protein 
      distribution in the syncytiotrophoblast remain unclear. We have previously 
      established rat syncytiotrophoblast cell lines, TR-TBT 18d-1 and TR-TBT 18d-2, 
      which retain characteristics of SynI and SynII, respectively. In this study, we 
      aimed to characterize the gene expression profiles in the two layers by using 
      these cell lines. DNA microarray analysis indicated that more than 25 mRNAs, 
      including cytoskeleton binding proteins, ezrin and CLP36, are differentially 
      expressed between TR-TBT 18d-1 and TR-TBT 18d-2. Quantitative real 
      time-polymerase chain reaction (PCR) analysis indicated that mRNA expression of 
      ezrin, CLP36, CCN1, and CCN2 is higher in TR-TBT 18d-1 and mRNA expression of 
      elf-1a, hsc70 and flot2 is higher in TR-TBT 18d-2, compared with their 
      counterparts. Immunohistochemical analysis indicated that ezrin is expressed in 
      rat placental villi in vivo, and is located on the apical membranes of TR-TBT 
      18d-1, while CLP36 is located in the apical and basal sides of TR-TBT 18d-1. The 
      expression of ezrin was highest at gestational days 14 and 18 and was highest 
      among the ezrin/radixin/moesin (ERM) family members. These results may help to 
      clarify the mechanisms controlling polarization of the syncytiotrophoblast and 
      the significance of the double epithelial layers in rat and mouse.
FAU - Higuchi, Kei
AU  - Higuchi K
AD  - Division of Pharmaceutics, Faculty of Pharmacy, Keio University, Tokyo, Japan.
FAU - Iizasa, Hisashi
AU  - Iizasa H
FAU - Sai, Yoshimichi
AU  - Sai Y
FAU - Horieya, Satomi
AU  - Horieya S
FAU - Lee, Kyeong-Eun
AU  - Lee KE
FAU - Wada, Masami
AU  - Wada M
FAU - Deguchi, Masayuki
AU  - Deguchi M
FAU - Nishimura, Tomohiro
AU  - Nishimura T
FAU - Wakayama, Tomohiko
AU  - Wakayama T
FAU - Tamura, Atsushi
AU  - Tamura A
FAU - Tsukita, Sachiko
AU  - Tsukita S
FAU - Kose, Noriko
AU  - Kose N
FAU - Kang, Young-Sook
AU  - Kang YS
FAU - Nakashima, Emi
AU  - Nakashima E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (LIM Domain Proteins)
RN  - 0 (Pdlim1 protein, rat)
RN  - 0 (ezrin)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cytoskeletal Proteins/*genetics
MH  - Female
MH  - *Gene Expression Profiling
MH  - Gestational Age
MH  - Homeodomain Proteins/*genetics
MH  - Immunohistochemistry
MH  - LIM Domain Proteins
MH  - Oligonucleotide Array Sequence Analysis
MH  - Pregnancy
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Trophoblasts/cytology/*metabolism
EDAT- 2010/08/06 06:00
MHDA- 2010/12/16 06:00
CRDT- 2010/08/06 06:00
PHST- 2010/08/06 06:00 [entrez]
PHST- 2010/08/06 06:00 [pubmed]
PHST- 2010/12/16 06:00 [medline]
AID - JST.JSTAGE/bpb/33.1400 [pii]
AID - 10.1248/bpb.33.1400 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2010;33(8):1400-6. doi: 10.1248/bpb.33.1400.