PMID- 20686384
OWN - NLM
STAT- MEDLINE
DCOM- 20101112
LR  - 20221207
IS  - 1525-1438 (Electronic)
IS  - 1048-891X (Linking)
VI  - 20
IP  - 4
DP  - 2010 May
TI  - Detection of human telomerase RNA gene in cervical cancer and precancerous 
      lesions: comparison with cytological and human papillomavirus DNA test findings.
PG  - 631-7
LID - 10.1111/IGC.0b013e3181c50c7e [doi]
AB  - OBJECTIVES: The aims of this study were to compare the findings of fluorescence 
      in situ hybridization (FISH) detection of human telomerase RNA gene (hTERC) 
      amplification with that of cytological and human papillomavirus (HPV) DNA tests 
      and explore the possibility to improve the accuracy of the diagnoses of 
      high-grade cervical intraepithelial neoplasia (CIN) and cervical cancer. METHODS: 
      A total of 201 specimens of liquid-based thin-layer cytological examination 
      findings were collected and detected by HPV DNA test and hTERC detected by FISH. 
      All women underwent colposcopy and histological examination of biopsy specimen if 
      needed. The 3 screening methods were compared based on histological examination 
      of colposcopic biopsies. RESULTS: The amplification of hTERC showed 6.06% in 
      normal or inflammation cases, 10.00% in CIN 1, 66.67% in CIN 2, 72.50% in CIN 3, 
      and 100.00% in carcinoma, with significant difference between the low- (<or=CIN 1 
      or <or=low-grade squamous intraepithelial lesion) and high-grade (>or=CIN 2 or 
      >or=atypical squamous cells in which high-grade squamous intraepithelial lesion 
      cannot be excluded) cervical lesions (P < 0.001). The hTERC amplification rate 
      was consistent with abnormal rates of cytological and histological diagnoses. The 
      detection of hTERC amplification had a much higher accuracy (87.56%) than the 
      cytological (80.10%) and HPV DNA test (77.61%) findings. CONCLUSIONS: Using FISH 
      to detect the amplification of hTERC had a much higher specificity and accuracy 
      for the diagnoses of high-grade CIN and cervical cancer than cytological and HPV 
      DNA test findings, suggesting that this detection might be a useful and specific 
      screening method in cervical cancer and precancerous lesions.
FAU - Li, Ying
AU  - Li Y
AD  - Women's Reproductive Health Laboratory of Zhejiang Province, School of Medicine, 
      Zhejiang University, Hangzhou, China.
FAU - Ye, Feng
AU  - Ye F
FAU - Lu, Wei-Guo
AU  - Lu WG
FAU - Zeng, Wen-Jie
AU  - Zeng WJ
FAU - Wei, Li-Hui
AU  - Wei LH
FAU - Xie, Xing
AU  - Xie X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Gynecol Cancer
JT  - International journal of gynecological cancer : official journal of the 
      International Gynecological Cancer Society
JID - 9111626
RN  - 0 (DNA, Neoplasm)
RN  - 0 (DNA, Viral)
RN  - 0 (telomerase RNA)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/*diagnosis/genetics/virology
MH  - *Cytodiagnosis
MH  - DNA, Neoplasm/genetics
MH  - DNA, Viral/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Papillomaviridae/genetics
MH  - Papillomavirus Infections/*diagnosis/genetics/virology
MH  - Polymerase Chain Reaction
MH  - Precancerous Conditions/*diagnosis/genetics/virology
MH  - Prognosis
MH  - RNA/*genetics
MH  - Telomerase/*genetics
MH  - Uterine Cervical Neoplasms/*diagnosis/genetics/virology
MH  - Vaginal Smears
MH  - Young Adult
MH  - Uterine Cervical Dysplasia/*diagnosis/genetics/virology
EDAT- 2010/08/06 06:00
MHDA- 2010/11/13 06:00
CRDT- 2010/08/06 06:00
PHST- 2010/08/06 06:00 [entrez]
PHST- 2010/08/06 06:00 [pubmed]
PHST- 2010/11/13 06:00 [medline]
AID - 00009577-201005000-00026 [pii]
AID - 10.1111/IGC.0b013e3181c50c7e [doi]
PST - ppublish
SO  - Int J Gynecol Cancer. 2010 May;20(4):631-7. doi: 10.1111/IGC.0b013e3181c50c7e.