PMID- 20686702
OWN - NLM
STAT- MEDLINE
DCOM- 20101028
LR  - 20220410
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 5
IP  - 7
DP  - 2010 Jul 29
TI  - The prepared tau exon-specific antibodies revealed distinct profiles of tau in 
      CSF of the patients with Creutzfeldt-Jakob disease.
PG  - e11886
LID - 10.1371/journal.pone.0011886 [doi]
LID - e11886
AB  - BACKGROUND: The diagnostic value of CSF tau for Creutzfeldt-Jakob disease (CJD) 
      has been widely evaluated, showing a markedly disease-relative manner. However, 
      the profiles of tau isoforms in CSF of CJD patients remain unknown. Here, we 
      prepared the exon-specific antibodies against the peptides encoded by exon-2, 
      exon-3 and exon-10 of human tau protein and evaluated the reactive profiles of 
      tau in CSF samples from the patients with probable CJD. METHODOLOGY/PRINCIPAL 
      FINDINGS: Sequences encoding exon-2, exon-3 and exon-10 of human tau protein were 
      cloned into a prokaryotic expression vector pGEX-2T. Using recombinant fusion 
      proteins GST-E2, GST-E3 and GST-E10, three tau exon-specific antibodies were 
      elicited. Reliable specificities of the prepared antibodies were obtained after a 
      serial of purification processes, not only in recognizing the tau peptides 
      encoded by exon-2, -3 and -10, but also in distinguishing six recombinant tau 
      isoforms by Western blot and ELISA. Three predominant tau-specific bands were 
      observed in CSF samples with the exon-specific and the commercial tau antibodies, 
      respectively, showing different reactive profiles between the groups of probable 
      CJD and non-CJD. A 65 KD band was detected only in the CSF samples from probable 
      CJD patients, especially with the antibodies against exon-2 (Anti-tE2) and 
      exon-10 (Anit-tE10). The appearances of 65 KD band in CSF correlated well with 
      positive 14-3-3 in CSF and typical abnormality in EEG. Such band was not observed 
      in the CSF samples of six tested genetic CJD patients. CONCLUSIONS/SIGNIFICANCE: 
      Three exon-specific polyclonal antibodies were successfully prepared. Based on 
      these antibodies, different CSF tau profiles in Western blots were observed 
      between the groups of probable CJD and non-CJD. A disease-specific tau band 
      emerged in the CSF samples from probable sporadic CJD, which may supply a new 
      biomarker for screening sporadic CJD.
FAU - Chen, Cao
AU  - Chen C
AD  - State Key Laboratory for Infectious Disease Prevention and Control, National 
      Institute for Viral Disease Control and Prevention, Chinese Center for Disease 
      Control and Prevention, Beijing, People's Republic of China.
FAU - Shi, Qi
AU  - Shi Q
FAU - Zhang, Bao-Yun
AU  - Zhang BY
FAU - Wang, Gui-Rong
AU  - Wang GR
FAU - Zhou, Wei
AU  - Zhou W
FAU - Gao, Chen
AU  - Gao C
FAU - Tian, Chan
AU  - Tian C
FAU - Mei, Guo-Yong
AU  - Mei GY
FAU - Han, Yan-Ling
AU  - Han YL
FAU - Han, Jun
AU  - Han J
FAU - Dong, Xiao-Ping
AU  - Dong XP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100729
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (14-3-3 Proteins)
RN  - 0 (Antibodies)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (tau Proteins)
SB  - IM
MH  - 14-3-3 Proteins/cerebrospinal fluid/immunology
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antibodies/*immunology
MH  - Antibody Specificity
MH  - Blotting, Western
MH  - Creutzfeldt-Jakob Syndrome/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Exons/*genetics
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Recombinant Fusion Proteins/genetics/immunology/metabolism
MH  - tau Proteins/*cerebrospinal fluid/*immunology
PMC - PMC2912377
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2010/08/06 06:00
MHDA- 2010/10/29 06:00
PMCR- 2010/07/29
CRDT- 2010/08/06 06:00
PHST- 2010/04/20 00:00 [received]
PHST- 2010/07/07 00:00 [accepted]
PHST- 2010/08/06 06:00 [entrez]
PHST- 2010/08/06 06:00 [pubmed]
PHST- 2010/10/29 06:00 [medline]
PHST- 2010/07/29 00:00 [pmc-release]
AID - 10-PONE-RA-18132R2 [pii]
AID - 10.1371/journal.pone.0011886 [doi]
PST - epublish
SO  - PLoS One. 2010 Jul 29;5(7):e11886. doi: 10.1371/journal.pone.0011886.