PMID- 20687229
OWN - NLM
STAT- MEDLINE
DCOM- 20100923
LR  - 20131121
IS  - 1097-0045 (Electronic)
IS  - 0270-4137 (Linking)
VI  - 70
IP  - 14
DP  - 2010 Oct 1
TI  - The effect of estrogen supplementation on cell proliferation and expression of 
      c-kit positive cells in the rat prostate.
PG  - 1555-62
LID - 10.1002/pros.21191 [doi]
AB  - BACKGROUND: Benign prostatic hyperplasia (BPH) is associated with the 
      proliferation of prostate tissue and an increase in smooth muscle tone. However, 
      the way in which the hormonal environment affects cell proliferation and 
      prostatic interstitial cells (PIC) responsible for the maintenance of the smooth 
      muscle tone is not clear. The present study investigated the effect of estrogen 
      supplementation on cell proliferation, androgen/estrogen ratio, and expression 
      and/or distribution of PIC. METHODS: Male Sprague-Dawley rats were anesthetized 
      with isoflurane/oxygen breathing mixture and subcutaneously implanted with 
      silastic capsules. These capsules were either empty or filled with a 10 or 20 mg 
      of crystalline estrogen. RESULTS: Estrogen exerted a potent effect on ventral 
      prostate weight, which was manifested as a significant decrease between controls 
      and the E(10)- and E(20)-treated rats. Active cell proliferation detected as 
      Ki67-positive nuclei was observed in the stromal and epithelial cells of the 
      ventral prostatic lobes from estrogen-treated rats and controls. Estrogen 
      supplementation caused a significant and dose-dependent increase in prostatic 
      estradiol and 5alpha-dihydrotestosterone (DHT) concentration but the ratios of 
      either DHT/E(2) or E(2)/DHT were not significantly affected. PIC were observed in 
      the region between the fibromuscular stroma and the glandular epithelium in all 
      three experimental groups. E(20)-treated rats showed a higher expression of PIC 
      than controls and E(10)-treated rats. CONCLUSIONS: The present study provides 
      novel information regarding the synergistic role of estrogens and androgens in 
      the prostate: estrogen may prevent prostatic hyperplasia via mechanisms other 
      than affecting cell proliferation or DHT/estrogen ratio.
CI  - (c) 2010 Wiley-Liss, Inc.
FAU - Kusljic, Snezana
AU  - Kusljic S
AD  - Department of Nursing, University of Melbourne, Carlton, Victoria, Australia.
FAU - Exintaris, Betty
AU  - Exintaris B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Prostate
JT  - The Prostate
JID - 8101368
RN  - 0 (Androgens)
RN  - 0 (Estrogens)
RN  - 0 (Ki-67 Antigen)
RN  - 08J2K08A3Y (Dihydrotestosterone)
RN  - 3XMK78S47O (Testosterone)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
SB  - IM
MH  - Androgens/metabolism/physiology
MH  - Animals
MH  - Body Weight/drug effects
MH  - Cell Division/drug effects
MH  - Dietary Supplements
MH  - Dihydrotestosterone/metabolism
MH  - Estradiol/metabolism
MH  - Estrogens/administration & dosage/metabolism/*pharmacology/physiology
MH  - Ki-67 Antigen/drug effects/metabolism
MH  - Leydig Cells/drug effects/physiology
MH  - Male
MH  - Organ Size/drug effects
MH  - Prostate/anatomy & histology/*cytology/drug effects/*physiology
MH  - Prostatic Hyperplasia/pathology
MH  - Proto-Oncogene Proteins c-kit/drug effects/*genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Testosterone/metabolism
EDAT- 2010/08/06 06:00
MHDA- 2010/09/24 06:00
CRDT- 2010/08/06 06:00
PHST- 2010/08/06 06:00 [entrez]
PHST- 2010/08/06 06:00 [pubmed]
PHST- 2010/09/24 06:00 [medline]
AID - 10.1002/pros.21191 [doi]
PST - ppublish
SO  - Prostate. 2010 Oct 1;70(14):1555-62. doi: 10.1002/pros.21191.