PMID- 20687453
OWN - NLM
STAT- MEDLINE
DCOM- 20100907
LR  - 20171116
IS  - 0143-2044 (Print)
IS  - 0143-2044 (Linking)
VI  - 31
IP  - 2
DP  - 2010 Mar-Apr
TI  - Cryopreservation of the human ovarian tissue induces the expression of Fas system 
      in morphologically normal primordial follicles.
PG  - 112-9
AB  - The aim of this study was to investigate whether the Fas system was involved in 
      cryopreservation process of the human ovarian tissue. Human ovarian cortical 
      tissues were cryopreserved using slow freezing method. Terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) was used to investigate 
      apoptosis of primordial follicle. Fas, Fas ligand and active caspase-3 expression 
      were detected by means of immunohistochemistry. The results showed that 
      immunostaining for Fas, Fas ligand and active caspase-3 were not detected in 
      morphologically normal primordial follicles in fresh ovarian tissue. After 
      cryopreservation, Fas, Fas ligand and active caspase-3 immunostaining were 
      present in morphologically normal primordial follicles. The study showed that 
      cryopreservation of the human ovarian tissue induced the expression of Fas system 
      in morphologically normal primordial follicles.
FAU - Xiao, Zhun
AU  - Xiao Z
AD  - Reproductive Medical Center of West China 2nd University Hospital, Sichuan 
      University, ChengDu 610041, China.
FAU - Wang, Yan
AU  - Wang Y
FAU - Li, Lei
AU  - Li L
FAU - Li, Shang-Wei
AU  - Li SW
LA  - eng
PT  - Journal Article
PL  - England
TA  - Cryo Letters
JT  - Cryo letters
JID - 9891832
RN  - 0 (FAS protein, human)
RN  - 0 (Fas Ligand Protein)
RN  - 0 (fas Receptor)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Adult
MH  - Apoptosis
MH  - Caspase 3/metabolism
MH  - *Cryopreservation
MH  - Fas Ligand Protein/*metabolism
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Ovarian Follicle/cytology/*metabolism
MH  - *Ovary
MH  - Young Adult
MH  - fas Receptor/*metabolism
EDAT- 2010/08/07 06:00
MHDA- 2010/09/08 06:00
CRDT- 2010/08/07 06:00
PHST- 2010/08/07 06:00 [entrez]
PHST- 2010/08/07 06:00 [pubmed]
PHST- 2010/09/08 06:00 [medline]
PST - ppublish
SO  - Cryo Letters. 2010 Mar-Apr;31(2):112-9.