PMID- 20688278
OWN - NLM
STAT- MEDLINE
DCOM- 20101202
LR  - 20141120
IS  - 1768-3238 (Electronic)
IS  - 1631-0691 (Linking)
VI  - 333
IP  - 8
DP  - 2010 Aug
TI  - In vitro interactions between bone marrow stromal cells and hippocampal slice 
      cultures.
PG  - 582-90
LID - 10.1016/j.crvi.2010.05.004 [doi]
AB  - Bone marrow stromal cells (BMSCs) are capable of differentiating into various 
      cell types including brain cells. Several groups have also demonstrated trophic 
      effects of MSC grafts in experimental ischemia models. However, the underlying 
      molecular mechanisms of these effects are not fully understood. We developed an 
      "in vitro graft model" which consisted in a coculture of GFP-expressing BMSCs and 
      hippocampal organotypic slice cultures. Total marrow cells (MCs) or BMSCs after 
      one (BMSC(1P)) or five passages (BMSC(5P)) were transplanted on hippocampal 
      slices. During the 10 days of our experiments, MCs and BMSC(1P) migrated toward 
      the tissue, but their total number remained constant. Conversely, the number of 
      BMSC(5P) decreased over the 10 days of the experiment, and no migration could be 
      detected. Using immunohistochemistry, we observed that the hippocampal slices 
      induced the expression of neural antigens in very few grafted cells, but MCs and 
      BMSC(1P) improved the conservation of the hippocampal slice culture. Similar 
      experiments using BMSC(5P) did not produce any significant change. We conclude 
      that the number of passages greatly influence BMSCs survival rate, migration and 
      neuroprotective capacities.
CI  - Copyright 2010 Academie des sciences. Published by Elsevier SAS. All rights 
      reserved.
FAU - Charriere, Karine
AU  - Charriere K
AD  - EA3922, IFR133/IBCT, universite de Franche-Comte, 19, rue Ambroise-Pare, 25000 
      Besancon, France. kcharrie@univ-fcomte.fr
FAU - Risold, Pierre-Yves
AU  - Risold PY
FAU - Fellmann, Dominique
AU  - Fellmann D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - C R Biol
JT  - Comptes rendus biologies
JID - 101140040
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/metabolism/*physiology
MH  - Bone Marrow Transplantation
MH  - Cells, Cultured
MH  - Green Fluorescent Proteins/metabolism
MH  - Hippocampus/cytology/metabolism/*physiology
MH  - Immunohistochemistry
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Organ Culture Techniques
MH  - Stromal Cells
MH  - Survival Analysis
EDAT- 2010/08/07 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/08/07 06:00
PHST- 2010/03/31 00:00 [received]
PHST- 2010/05/20 00:00 [revised]
PHST- 2010/05/24 00:00 [accepted]
PHST- 2010/08/07 06:00 [entrez]
PHST- 2010/08/07 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - S1631-0691(10)00167-8 [pii]
AID - 10.1016/j.crvi.2010.05.004 [doi]
PST - ppublish
SO  - C R Biol. 2010 Aug;333(8):582-90. doi: 10.1016/j.crvi.2010.05.004.