PMID- 20689056
OWN - NLM
STAT- MEDLINE
DCOM- 20101028
LR  - 20220317
IS  - 1522-1547 (Electronic)
IS  - 0193-1857 (Print)
IS  - 0193-1857 (Linking)
VI  - 299
IP  - 4
DP  - 2010 Oct
TI  - Hypothalamic oxytocin mediates adaptation mechanism against chronic stress in 
      rats.
PG  - G946-53
LID - 10.1152/ajpgi.00483.2009 [doi]
AB  - Accumulation of continuous life stress (chronic stress) often causes gastric 
      symptoms. Although central oxytocin has antistress effects, the role of central 
      oxytocin in stress-induced gastric dysmotility remains unknown. Solid gastric 
      emptying was measured in rats receiving acute restraint stress, 5 consecutive 
      days of repeated restraint stress (chronic homotypic stress), and 7 consecutive 
      days of varying types of stress (chronic heterotypic stress). Oxytocin and 
      oxytocin receptor antagonist were administered intracerebroventricularly (icv). 
      Expression of corticotropin-releasing factor (CRF) mRNA and oxytocin mRNA in the 
      paraventricular nucleus (PVN) of the hypothalamus was evaluated by real-time 
      RT-PCR. The changes of oxytocinergic neurons in the PVN were evaluated by 
      immunohistochemistry. Acute stress delayed gastric emptying, and the delayed 
      gastric emptying was completely restored after 5 consecutive days of chronic 
      homotypic stress. In contrast, delayed gastric emptying persisted following 
      chronic heterotypic stress. The restored gastric emptying following chronic 
      homotypic stress was antagonized by icv injection of an oxytocin antagonist. Icv 
      injection of oxytocin restored delayed gastric emptying induced by chronic 
      heterotypic stress. CRF mRNA expression, which was significantly increased in 
      response to acute stress and chronic heterotypic stress, returned to the basal 
      levels following chronic homotypic stress. In contrast, oxytocin mRNA expression 
      was significantly increased following chronic homotypic stress. The number of 
      oxytocin-immunoreactive cells was increased following chronic homotypic stress at 
      the magnocellular part of the PVN. Icv injection of oxytocin reduced CRF mRNA 
      expression induced by acute stress and chronic heterotypic stress. It is 
      suggested that the adaptation mechanism to chronic stress may involve the 
      upregulation of oxytocin expression in the hypothalamus, which in turn attenuates 
      CRF expression.
FAU - Zheng, Jun
AU  - Zheng J
AD  - Department of Surgery, Medical College of Wisconsin, Zablocki Veterans Affairs 
      Medical Center, Milwaukee, USA.
FAU - Babygirija, Reji
AU  - Babygirija R
FAU - Bulbul, Mehmet
AU  - Bulbul M
FAU - Cerjak, Diana
AU  - Cerjak D
FAU - Ludwig, Kirk
AU  - Ludwig K
FAU - Takahashi, Toku
AU  - Takahashi T
LA  - eng
GR  - R01 DK62768/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100805
PL  - United States
TA  - Am J Physiol Gastrointest Liver Physiol
JT  - American journal of physiology. Gastrointestinal and liver physiology
JID - 100901227
RN  - 0 (Oxytocics)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Oxytocin)
RN  - 34330-23-9 (tocinoic acid)
RN  - 50-56-6 (Oxytocin)
SB  - IM
MH  - Animals
MH  - Brain/cytology/metabolism
MH  - Brain Chemistry
MH  - Chronic Disease
MH  - Gene Expression Regulation/physiology
MH  - Hypothalamus/*metabolism
MH  - Immunohistochemistry
MH  - Injections, Intraventricular
MH  - Male
MH  - Oxytocics/administration & dosage/antagonists & inhibitors/*pharmacology
MH  - Oxytocin/administration & dosage/analogs & derivatives/metabolism/*pharmacology
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Oxytocin/antagonists & inhibitors
MH  - Restraint, Physical
MH  - Stress, Physiological/*physiology
PMC - PMC2957337
EDAT- 2010/08/07 06:00
MHDA- 2010/10/29 06:00
PMCR- 2011/10/01
CRDT- 2010/08/07 06:00
PHST- 2010/08/07 06:00 [entrez]
PHST- 2010/08/07 06:00 [pubmed]
PHST- 2010/10/29 06:00 [medline]
PHST- 2011/10/01 00:00 [pmc-release]
AID - ajpgi.00483.2009 [pii]
AID - GI-00483-2009 [pii]
AID - 10.1152/ajpgi.00483.2009 [doi]
PST - ppublish
SO  - Am J Physiol Gastrointest Liver Physiol. 2010 Oct;299(4):G946-53. doi: 
      10.1152/ajpgi.00483.2009. Epub 2010 Aug 5.