PMID- 20690059
OWN - NLM
STAT- MEDLINE
DCOM- 20101228
LR  - 20100806
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 664
DP  - 2010
TI  - DNA copy number analysis on tissue microarrays.
PG  - 127-34
LID - 10.1007/978-1-60761-806-5_13 [doi]
AB  - Detection of DNA sequence copy number changes is essential in both clinical 
      practice and basic research, especially in cancer research. The combination of 
      fluorescence in situ hybridization (FISH) and tissue microarray (TMA) technology 
      provides high-throughput means for the evaluation of genetic aberrations in a 
      large number of tissue samples. FISH on TMA is technically demanding and several 
      protocols that include a variety of tissue pretreatment steps have been developed 
      to improve the success of this methodology. Despite of the technical 
      difficulties, FISH analysis on TMA has been successfully used not only to uncover 
      genetic alterations in various malignancies but to also rapidly establish the 
      clinical significance of such changes.
FAU - Kallioniemi, Anne
AU  - Kallioniemi A
AD  - Institute of Medical Technology, University of Tampere and Tampere University 
      Hospital, Tampere, Finland. anne.kallioniemi@uta.fi
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Fluorescent Dyes)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA/*genetics/metabolism
MH  - Fluorescent Dyes/metabolism
MH  - *Gene Dosage
MH  - In Situ Hybridization, Fluorescence
MH  - Tissue Array Analysis/*methods
EDAT- 2010/08/07 06:00
MHDA- 2010/12/29 06:00
CRDT- 2010/08/07 06:00
PHST- 2010/08/07 06:00 [entrez]
PHST- 2010/08/07 06:00 [pubmed]
PHST- 2010/12/29 06:00 [medline]
AID - 10.1007/978-1-60761-806-5_13 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;664:127-34. doi: 10.1007/978-1-60761-806-5_13.