PMID- 20690062
OWN - NLM
STAT- MEDLINE
DCOM- 20101228
LR  - 20100806
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 664
DP  - 2010
TI  - Digital microscopy for boosting database integration and analysis in TMA studies.
PG  - 163-75
LID - 10.1007/978-1-60761-806-5_16 [doi]
AB  - The enormous amount of clinical, pathological, and staining data to be linked, 
      analyzed, and correlated in a tissue microarray (TMA) project makes digital 
      slides ideal to be integrated into TMA database systems. With the help of a 
      computer and dedicated software tools, digital slides offer dynamic access to 
      microscopic information at any magnification with easy navigation, annotation, 
      measurement, and archiving features. Advanced slide scanners work both in 
      transmitted light and fluorescent modes to support biomarker testing with 
      immunohistochemistry, immunofluorescence or fluorescence in situ hybridization 
      (FISH). Currently, computer-driven integrated systems are available for creating 
      TMAs, digitalizing TMA slides, linking sample and staining data, and analyzing 
      their results. Digital signals permit image segmentation along color, intensity, 
      and size for automated object quantification where digital slides offer superior 
      imaging features and batch processing. In this chapter, the workflow and the 
      advantages of digital TMA projects are demonstrated through the project-based 
      MIRAX system developed by 3DHISTECH and supported by Zeiss.The enhanced features 
      of digital slides compared with those of still images can boost integration and 
      intelligence in TMA database management systems, offering essential support for 
      high-throughput biomarker testing, for example, in tumor progression/prognosis, 
      drug discovery, and target therapy research.
FAU - Krenacs, Tibor
AU  - Krenacs T
AD  - Department of Pathology and Experimental Cancer Research, Budapest, Hungary. 
      krenacst@gmail.com
FAU - Ficsor, Levente
AU  - Ficsor L
FAU - Varga, Sebestyen Viktor
AU  - Varga SV
FAU - Angeli, Vivien
AU  - Angeli V
FAU - Molnar, Bela
AU  - Molnar B
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - *Databases, Factual
MH  - Microscopy/*methods
MH  - Software
MH  - Statistics as Topic
MH  - *Systems Integration
MH  - Tissue Array Analysis/*methods
EDAT- 2010/08/07 06:00
MHDA- 2010/12/29 06:00
CRDT- 2010/08/07 06:00
PHST- 2010/08/07 06:00 [entrez]
PHST- 2010/08/07 06:00 [pubmed]
PHST- 2010/12/29 06:00 [medline]
AID - 10.1007/978-1-60761-806-5_16 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;664:163-75. doi: 10.1007/978-1-60761-806-5_16.