PMID- 20690503
OWN - NLM
STAT- MEDLINE
DCOM- 20100914
LR  - 20211020
IS  - 1674-2818 (Print)
IS  - 2049-3169 (Electronic)
IS  - 1674-2818 (Linking)
VI  - 1
IP  - 1
DP  - 2009 Mar
TI  - Sequential fluorescent labeling observation of maxillary sinus augmentation by a 
      tissue-engineered bone complex in canine model.
PG  - 39-46
LID - 10.4248/ijos.08022 [doi]
AB  - AIM: To evaluate the effects of maxillary sinus floor elevation by a 
      tissue-engineered bone complex of beta-tricalcium phosphate (beta-TCP) and 
      autologous osteoblasts in dogs. METHODOLOGY: Autologous osteoblasts from adult 
      Beagle dogs were cultured in vitro. They were further combined with beta-TCP to 
      construct the tissue-engineered bone complex. 12 cases of maxillary sinus floor 
      elevation surgery were made bilaterally in 6 animals and randomly repaired with 
      the following 3 groups of materials: Group A (osteoblasts/beta-TCP); Group B 
      (beta-TCP); Group C (autogenous bone) (n=4 per group). A polychrome sequential 
      fluorescent labeling was performed post-operatively and the animals were 
      sacrificed 24 weeks after operation for histological observation. RESULTS: Our 
      results showed that autologous osteoblasts were successfully expanded and the 
      osteoblastic phenol-types were confirmed by ALP and Alizarin red staining. The 
      cells could attach and proliferate well on the surface of the beta-TCP scaffold. 
      The fluorescent and histological observation showed that the tissue-engineered 
      bone complex had an earlier mineralization and more bone formation inside the 
      scaffold than beta-TCP along or even autologous bone. It had also maximally 
      maintained the elevated sinus height than both control groups. CONCLUSION: Porous 
      beta-TCP has served as a good scaffold for autologous osteoblasts seeding. The 
      tissue-engineered bone complex with beta-TCP and autologous osteoblasts might be 
      a better alternative to autologous bone for the clinical edentulous maxillary 
      sinus augmentation.
FAU - Jiang, Xin-quan
AU  - Jiang XQ
AD  - Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of 
      Stomatolgoy, Shanghai Ninth People's Hospital affiliated to Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
FAU - Wang, Shao-yi
AU  - Wang SY
FAU - Zhao, Jun
AU  - Zhao J
FAU - Zhang, Xiu-li
AU  - Zhang XL
FAU - Zhang, Zhi-yuan
AU  - Zhang ZY
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Int J Oral Sci
JT  - International journal of oral science
JID - 101504351
RN  - 0 (Anthraquinones)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Biomarkers)
RN  - 0 (Bone Substitutes)
RN  - 0 (Calcium Phosphates)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (beta-tricalcium phosphate)
RN  - 60MEW57T9G (alizarin)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/analysis
MH  - Alveolar Ridge Augmentation/*methods
MH  - Animals
MH  - Anthraquinones
MH  - Biocompatible Materials/*therapeutic use
MH  - Biomarkers/analysis
MH  - Bone Substitutes/therapeutic use
MH  - Bone Transplantation/pathology
MH  - Calcification, Physiologic/physiology
MH  - Calcium Phosphates/*therapeutic use
MH  - Cell Adhesion/physiology
MH  - Cell Proliferation
MH  - Dogs
MH  - Fluorescent Dyes
MH  - Guided Tissue Regeneration, Periodontal/methods
MH  - Maxilla/*surgery
MH  - Maxillary Sinus/*surgery
MH  - Models, Animal
MH  - Osteoblasts/*transplantation
MH  - Osteogenesis/physiology
MH  - Random Allocation
MH  - Tissue Engineering/*methods
MH  - *Tissue Scaffolds
MH  - Transplantation, Autologous
PMC - PMC3735791
EDAT- 2009/03/01 00:00
MHDA- 2010/09/16 06:00
PMCR- 2009/03/01
CRDT- 2010/08/10 06:00
PHST- 2010/08/10 06:00 [entrez]
PHST- 2009/03/01 00:00 [pubmed]
PHST- 2010/09/16 06:00 [medline]
PHST- 2009/03/01 00:00 [pmc-release]
AID - ijos20098 [pii]
AID - 10.4248/ijos.08022 [doi]
PST - ppublish
SO  - Int J Oral Sci. 2009 Mar;1(1):39-46. doi: 10.4248/ijos.08022.