PMID- 20690791
OWN - NLM
STAT- MEDLINE
DCOM- 20101129
LR  - 20181201
IS  - 1464-5246 (Electronic)
IS  - 0265-2048 (Linking)
VI  - 27
IP  - 6
DP  - 2010
TI  - Controlled release of bioactive recombinant human growth hormone from PLGA 
      microparticles.
PG  - 552-60
LID - 10.3109/02652048.2010.489974 [doi]
AB  - Controlled release formulation of recombinant human growth hormone (r-hGH) was 
      achieved using poly lactide-co-glycolide (PLGA) polymer. Denaturation of r-hGH by 
      dichloromethane during primary emulsification step of particle preparation was 
      minimized by using human serum albumin whereas inclusion of sucrose and sodium 
      bicarbonate helped in reducing protein denaturation during lyophilization and 
      polymer particle degradation. Encapsulation efficiency of r-hGH entrapped in PLGA 
      particles (size approximately 30 microm) was around 45% with protein load 20 
      microg of r-hGH/mg of polymer particles. Porous particles showed quick release of 
      r-hGH in comparison to non-porous particles in vitro. More than 10 ng/mL of 
      bioactive r-hGH was found in the serum of the experimental animals observed for a 
      30-day period after a single intramuscular injection of the polymeric 
      formulation. Incorporation of optimal stabilizers is thus essential for the 
      development of a stable, month long controlled release of polymer particle based 
      r-hGH formulation.
FAU - Rafi, M
AU  - Rafi M
AD  - Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, 
      New Delhi 110067, India.
FAU - Singh, Surinder M
AU  - Singh SM
FAU - Kanchan, Vibhu
AU  - Kanchan V
FAU - Anish, C K
AU  - Anish CK
FAU - Panda, Amulya K
AU  - Panda AK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Microencapsul
JT  - Journal of microencapsulation
JID - 8500513
RN  - 0 (Delayed-Action Preparations)
RN  - 0 (Recombinant Proteins)
RN  - 12629-01-5 (Human Growth Hormone)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Delayed-Action Preparations/*chemistry
MH  - Drug Compounding
MH  - Freeze Drying
MH  - Human Growth Hormone/*administration & dosage/blood/chemistry/*pharmacology
MH  - Humans
MH  - Lactic Acid/*chemistry
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Protein Denaturation
MH  - Protein Stability
MH  - Rats
MH  - Rats, Wistar
MH  - Recombinant Proteins/administration & dosage/blood/chemistry/pharmacology
EDAT- 2010/08/10 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/08/10 06:00
PHST- 2010/08/10 06:00 [entrez]
PHST- 2010/08/10 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - 10.3109/02652048.2010.489974 [doi]
PST - ppublish
SO  - J Microencapsul. 2010;27(6):552-60. doi: 10.3109/02652048.2010.489974.