PMID- 20691082
OWN - NLM
STAT- MEDLINE
DCOM- 20101028
LR  - 20231213
IS  - 1756-9966 (Electronic)
IS  - 0392-9078 (Print)
IS  - 0392-9078 (Linking)
VI  - 29
IP  - 1
DP  - 2010 Aug 6
TI  - HSP70: a promising target for laryngeal carcinoma radiaotherapy by inhibiting 
      cleavage and degradation of nucleolin.
PG  - 106
LID - 10.1186/1756-9966-29-106 [doi]
AB  - Previous studies have shown that heat shock proteins (HSPs) were upregulated in 
      various types of tumors and were associated with histological grade, recurrence 
      and metastasis of malignant tumors. In this study, we investigated whether heat 
      shock protein 70 kDa (HSP70) was associated with histological grade of laryngeal 
      squamous cell carcinomas (LSCC). We also determine the role of HSP70 in LSCC 
      radiation resistance using a laryngeal carcinoma xenograft model by antisense 
      HSP70 RNA technique. Immunohistochemistry data showed that HSP70 was detected in 
      96% of LSCC tissues (48 out of 50). The expression level of HSP70 was 
      significantly lower in early stage of LSCC than that in late stage (P = 0.015). 
      Radiation treatment result showed that the volumes and weights of implantation 
      tumors in the group injected with antisense HSP70 oligos were significantly 
      reduced comparing to the group injected with random oligos(p < 0.05). In 
      addition, cleavage and degradation of tumor nucleolin in antisense HSP70 oligos 
      injection group was significantly higher than that in random oligos injection 
      group. Our result suggested that HSP70 may play a role in LSCC radiotherapy 
      resistance by inhibiting cleavage and degradation of nucleolin.
FAU - Xu, Jing
AU  - Xu J
AD  - Department of Otolaryngology, Central South University, Changsha, People's 
      Republic of China.
FAU - Wang, Kangkai
AU  - Wang K
FAU - Zhang, Xin
AU  - Zhang X
FAU - Qiu, Yuanzheng
AU  - Qiu Y
FAU - Huang, Donghai
AU  - Huang D
FAU - Li, Wei
AU  - Li W
FAU - Xiao, Xianzhong
AU  - Xiao X
FAU - Tian, Yongquan
AU  - Tian Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100806
PL  - England
TA  - J Exp Clin Cancer Res
JT  - Journal of experimental & clinical cancer research : CR
JID - 8308647
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Apoptosis/radiation effects
MH  - Blotting, Western
MH  - Carcinoma, Squamous Cell/*metabolism/pathology/*radiotherapy
MH  - Female
MH  - HSP70 Heat-Shock Proteins/antagonists & inhibitors/genetics/*metabolism
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - In Situ Nick-End Labeling
MH  - Laryngeal Neoplasms/*metabolism/pathology/*radiotherapy
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Oligonucleotides, Antisense/pharmacology
MH  - Phosphoproteins/genetics/*metabolism
MH  - RNA, Messenger/genetics
MH  - RNA-Binding Proteins/genetics/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tissue Array Analysis
MH  - Tumor Cells, Cultured
MH  - Nucleolin
PMC - PMC2928197
EDAT- 2010/08/10 06:00
MHDA- 2010/10/29 06:00
PMCR- 2010/08/06
CRDT- 2010/08/10 06:00
PHST- 2010/04/29 00:00 [received]
PHST- 2010/08/06 00:00 [accepted]
PHST- 2010/08/10 06:00 [entrez]
PHST- 2010/08/10 06:00 [pubmed]
PHST- 2010/10/29 06:00 [medline]
PHST- 2010/08/06 00:00 [pmc-release]
AID - 1756-9966-29-106 [pii]
AID - 10.1186/1756-9966-29-106 [doi]
PST - epublish
SO  - J Exp Clin Cancer Res. 2010 Aug 6;29(1):106. doi: 10.1186/1756-9966-29-106.