PMID- 20691696
OWN - NLM
STAT- MEDLINE
DCOM- 20101007
LR  - 20171116
IS  - 1872-7905 (Electronic)
IS  - 0022-1759 (Linking)
VI  - 361
IP  - 1-2
DP  - 2010 Sep 30
TI  - Neutrophil activation during transmigration in vivo and in vitro A translational 
      study using the skin chamber model.
PG  - 82-8
LID - 10.1016/j.jim.2010.07.015 [doi]
AB  - Neutrophil transmigration can be studied in vitro by use of the transwell model 
      and in vivo by the skin chamber model. Activation during transmigration involves 
      translocation of secretory vesicles and granules to the plasma- and phagolysosome 
      membranes. In this study, we compared the skin chamber model with the transwell 
      model, focusing on the mobilization of CR1 (CD35), CR3 (CD11b/CD18) and CD63 from 
      intracellular vesicles and granules. In addition, functional responses towards a 
      bacterial related stimulus, formyl-methionyl-leucyl-phenylalanine (fMLP), in 
      terms of CR3 expression and production of reactive oxygen species (ROS) were 
      assessed. Discrepancies between the skin chamber model and the transwell model 
      were observed. The expression of CR1 increased following in vivo transmigration 
      (p<0.001) and, in contrast, decreased following in vitro transmigration 
      (p=0.004). Furthermore, CR1 was mobilized following an isolation procedure 
      included in the transwell model. The expression of CR3 increased following both 
      in vivo (p<0.001) and in vitro (p=0.03) transmigration. However, in vitro 
      transmigration did not influence the fMLP induced CR3 expression which was 
      significantly increased following in vivo transmigration (p=0.01). In addition, 
      the fMLP induced production of ROS was significantly reduced following in vitro 
      transmigration (p=0.002) but unaltered after in vivo transmigration, indicating 
      differences between the impact of the two systems on cellular activation. The 
      observed discrepancies between the two models might be partly explained by 
      granule mobilization and neutrophil priming, induced during the isolation 
      procedure included in the transwell model, which results in an altered cellular 
      activation. Therefore, mobilization of granules needs to be accounted for when 
      interpreting data from different model systems.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Paulsson, Josefin M
AU  - Paulsson JM
AD  - Department of Clinical Immunology, Karolinska Institutet, Karolinska University 
      Hospital, Stockholm, Sweden. josefin.paulsson@ki.se
FAU - Jacobson, Stefan H
AU  - Jacobson SH
FAU - Lundahl, Joachim
AU  - Lundahl J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100805
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD1)
RN  - 0 (CD1b antigen)
RN  - 0 (CD63 protein, human)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Receptors, Complement 3b)
RN  - 0 (Tetraspanin 30)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Antigens, CD/immunology
MH  - Antigens, CD1/immunology
MH  - Apoptosis/*immunology
MH  - Cell Movement/*immunology
MH  - Cytoplasmic Granules/*immunology/ultrastructure
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Male
MH  - Microscopy, Electron, Transmission
MH  - Middle Aged
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophil Activation/*immunology
MH  - Neutrophils/*immunology/ultrastructure
MH  - Platelet Membrane Glycoproteins/immunology
MH  - Reactive Oxygen Species/immunology
MH  - Receptors, Complement 3b/immunology
MH  - Secretory Vesicles/*immunology/ultrastructure
MH  - Tetraspanin 30
MH  - Young Adult
EDAT- 2010/08/10 06:00
MHDA- 2010/10/12 06:00
CRDT- 2010/08/10 06:00
PHST- 2010/05/21 00:00 [received]
PHST- 2010/07/07 00:00 [revised]
PHST- 2010/07/29 00:00 [accepted]
PHST- 2010/08/10 06:00 [entrez]
PHST- 2010/08/10 06:00 [pubmed]
PHST- 2010/10/12 06:00 [medline]
AID - S0022-1759(10)00211-5 [pii]
AID - 10.1016/j.jim.2010.07.015 [doi]
PST - ppublish
SO  - J Immunol Methods. 2010 Sep 30;361(1-2):82-8. doi: 10.1016/j.jim.2010.07.015. 
      Epub 2010 Aug 5.