PMID- 20692146
OWN - NLM
STAT- MEDLINE
DCOM- 20110331
LR  - 20191210
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 26
IP  - 4
DP  - 2010 Dec 15
TI  - Detection of beta-amyloid peptide (1-16) and amyloid precursor protein (APP770) 
      using spectroscopic ellipsometry and QCM techniques: a step forward towards 
      Alzheimers disease diagnostics.
PG  - 1332-6
LID - 10.1016/j.bios.2010.07.042 [doi]
AB  - A highly sensitive method of spectroscopic ellipsometry in total internal 
      reflection mode (TIRE) was exploited for detecting beta-amyloid peptide (Abeta(1-16)) 
      in the direct immune reaction with monoclonal DE2 antibodies (raised against 
      Abeta(1-16)) electrostatically immobilised on the surface of gold. A rapid detection 
      of Abeta(1-16) in a wide range of concentrations from 5 mug/ml down to 0.05 ng/ml was 
      achieved using a cost-effective and label-free direct immunoassay format. TIRE 
      dynamic spectral measurements proved that the immune reaction between DE2 
      monoclonal antibodies and Abeta(1-16) is highly specific with the affinity constant 
      K(D)=1.46x10(-8) mol/l. The same DE2 antibodies were utilised for detection of 
      amyloid precursor protein APP(770), a larger protein containing Abeta(1-16) domain, 
      using the quartz crystal microbalance (QCM) measurements in liquid. A combination 
      of QCM and TIRE kinetics results allowed the evaluation of the originally unknown 
      concentration of APP(770) in complete medium solution containing other proteins, 
      salts, and amino acids.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Mustafa, M K
AU  - Mustafa MK
AD  - Materials and Engineering Research Institute, Sheffield Hallam University, Howard 
      Street, S1 1WB, UK. m.k.mustafa@shu.ac.uk
FAU - Nabok, A
AU  - Nabok A
FAU - Parkinson, D
AU  - Parkinson D
FAU - Tothill, I E
AU  - Tothill IE
FAU - Salam, F
AU  - Salam F
FAU - Tsargorodskaya, A
AU  - Tsargorodskaya A
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100718
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Antibodies, Immobilized)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Peptide Fragments)
RN  - 0 (amyloid beta-protein (1-16))
SB  - IM
MH  - Alzheimer Disease/*diagnosis/*metabolism
MH  - Amyloid beta-Peptides/*analysis/immunology
MH  - Amyloid beta-Protein Precursor/*analysis/immunology
MH  - Animals
MH  - Antibodies, Immobilized
MH  - Antibodies, Monoclonal/metabolism
MH  - Antigen-Antibody Reactions
MH  - Humans
MH  - Immunoassay/methods
MH  - In Vitro Techniques
MH  - Kinetics
MH  - Mice
MH  - Peptide Fragments/*analysis/immunology
MH  - Quartz Crystal Microbalance Techniques/*methods/statistics & numerical data
MH  - Spectrum Analysis/methods
MH  - Surface Plasmon Resonance/methods
EDAT- 2010/08/10 06:00
MHDA- 2011/04/01 06:00
CRDT- 2010/08/10 06:00
PHST- 2010/04/19 00:00 [received]
PHST- 2010/07/02 00:00 [revised]
PHST- 2010/07/13 00:00 [accepted]
PHST- 2010/08/10 06:00 [entrez]
PHST- 2010/08/10 06:00 [pubmed]
PHST- 2011/04/01 06:00 [medline]
AID - S0956-5663(10)00417-3 [pii]
AID - 10.1016/j.bios.2010.07.042 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2010 Dec 15;26(4):1332-6. doi: 10.1016/j.bios.2010.07.042. 
      Epub 2010 Jul 18.