PMID- 20693704
OWN - NLM
STAT- MEDLINE
DCOM- 20110804
LR  - 20201209
IS  - 1672-7347 (Print)
IS  - 1672-7347 (Linking)
VI  - 35
IP  - 7
DP  - 2010 Jul
TI  - Interaction between nuclear localization signal-retinoic acid receptor alpha and 
      Ubiquilin 1.
PG  - 649-54
LID - 10.3969/j.issn.1672-7347.2010.07.002 [doi]
AB  - OBJECTIVE: To identify the interaction between nuclear localization 
      signal-retinoic acid receptor alpha (NLS-RARalpha) and Ubiquilin 1(UBQLN1). 
      METHODS: The recombination expression plasmids pGBKT7-NLS-RAR and pACT2-UBQLN1, 
      which expressed bait-protein NLS-RARalpha and target protein UBQLN1 respectively, 
      were cotransformated into yeast AH109. The interaction of the expression plasmids 
      in the living cells was investigated by yeast two-hybrid assay. HA-tagged fusion 
      protein (pCMV-HA-NLS-RARalpha) expression vector and Myc-tagged fusion protein 
      (pCMV-Myc-JTV1) expression vector were constructed, identified, and cotransfected 
      respectively into human embryo kidney 293 cells(HEK293). The interaction was 
      detected by co-immunoprecipitation. RESULTS: Blue clones were found on yeast 
      AH109 plate cotransformated with pGBKT7-NLS-RARalpha and pACT2-UBQLN1. Double 
      restriction enzyme digestion showed that pCMV-HA-NLS-RARalpha and pCMV-Myc-JTV1 
      were successfully constructed. Then HA-NLS-RAR protein was immunoprecipitated by 
      anti-HA polyclonal antibody and the expression of Myc-UBQLN1 was tested by 
      Western blot with anti-Myc monoclonal antibody from immunoprecipitated complex. 
      CONCLUSION: The interaction between NLS-RARalpha and UBQLN1 can be verified by 
      yeast two-hybrid assay and co-immunoprecipitation.
FAU - Zhu, Dan
AU  - Zhu D
AD  - Key Laboratory of Laboratory Medicine Diagnostics, Ministry of Education of 
      People's Repulic of China, Faculty of Laboratory Medicine, Chongqing Medical 
      University, Chongqing 400016, China.
FAU - Wang, Chong
AU  - Wang C
FAU - Liu, Beizhong
AU  - Liu B
FAU - Wu, Yan
AU  - Wu Y
FAU - Zhong, Liang
AU  - Zhong L
FAU - Wang, Chunguang
AU  - Wang C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhong Nan Da Xue Xue Bao Yi Xue Ban
JT  - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. 
      Medical sciences
JID - 101230586
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Autophagy-Related Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Nuclear Localization Signals)
RN  - 0 (RARA protein, human)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoic Acid Receptor alpha)
RN  - 0 (UBQLN1 protein, human)
SB  - IM
MH  - Active Transport, Cell Nucleus
MH  - Adaptor Proteins, Signal Transducing
MH  - Autophagy-Related Proteins
MH  - Base Sequence
MH  - Carrier Proteins/genetics/*metabolism
MH  - Cell Cycle Proteins/genetics/*metabolism
MH  - HEK293 Cells
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nuclear Localization Signals/genetics/*metabolism
MH  - Protein Interaction Domains and Motifs/*physiology
MH  - Receptors, Retinoic Acid/genetics/*metabolism
MH  - Retinoic Acid Receptor alpha
MH  - Transfection
MH  - Two-Hybrid System Techniques
EDAT- 2010/08/10 06:00
MHDA- 2011/08/05 06:00
CRDT- 2010/08/10 06:00
PHST- 2010/08/10 06:00 [entrez]
PHST- 2010/08/10 06:00 [pubmed]
PHST- 2011/08/05 06:00 [medline]
AID - 10.3969/j.issn.1672-7347.2010.07.002 [doi]
PST - ppublish
SO  - Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2010 Jul;35(7):649-54. doi: 
      10.3969/j.issn.1672-7347.2010.07.002.