PMID- 20695081
OWN - NLM
STAT- MEDLINE
DCOM- 20100914
LR  - 20211020
IS  - 1674-2818 (Print)
IS  - 2049-3169 (Electronic)
IS  - 1674-2818 (Linking)
VI  - 1
IP  - 3
DP  - 2009 Sep
TI  - Fluconazole susceptibility and genotypic heterogeneity of oral Candida albicans 
      colonies from the patients with cancer receiving chemotherapy in China.
PG  - 156-62
LID - 10.4248/IJOS.09020 [doi]
AB  - AIM: To identify heterogeneity of Candida albicans (C. albicans) isolated from 
      the population with cancer in China by using identification medium, subculture 
      molecular typing, and antifungal susceptibility test. METHODOLOGY: Oral cheek 
      mucosal specimens from 52 cancer patients receiving chemotherapy were cultured on 
      CHROMagar Candida plates for Candida identification. All the C. albicans colonies 
      on the plates were subcultured and reconfirmed by API20C, then submitted to the 
      antifungal drug susceptibility test with fluconazole and molecular typing using 
      randomly amplified polymorphic DNA-PCR (RAPD) with primers RSD6 and RSD12. 
      RESULTS: 54% (28/52) patients were oral yeast carriage in which C. albicans 
      predominated. More than 7 C. albicans colonies were isolated from each of 12 
      patients (Group A), while less than 5 colonies were isolated from each of 16 
      patients (Group B). RSD6 and RSD12 were successful in eliciting 17 (A1-A17) and 2 
      (B1-B2) genotypes, respectively from among the 205 isolates. The two primers were 
      combined to generate 21 genotypes. The C. albicans isolates obtained from the 
      same patient and episode showed a diversity for fluconazole revealed by MIC50 and 
      MIC90. CONCLUSION: The heterogeneity of the C. albicans colonies isolated from 
      the same patients can be detected. C. albicans with varied fluconazole 
      susceptibility and genotypic characteristics may coexist in the same oral Candida 
      population.
FAU - Sun, Jing
AU  - Sun J
AD  - Department of Oral Medicine, Key Lab of Oral Biomedicine, Shandong Province, 
      School of Stomatology, Shandong University, Jinan, China.
FAU - Qi, Cheng
AU  - Qi C
FAU - Lafleur, Micheal D
AU  - Lafleur MD
FAU - Qi, Qing-guo
AU  - Qi QG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Int J Oral Sci
JT  - International journal of oral science
JID - 101504351
RN  - 0 (Antifungal Agents)
RN  - 0 (DNA, Fungal)
RN  - 8VZV102JFY (Fluconazole)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antifungal Agents/*pharmacology
MH  - Candida albicans/*classification/genetics/isolation & purification
MH  - Candida glabrata/classification/isolation & purification
MH  - Candidiasis, Oral/microbiology
MH  - China
MH  - DNA, Fungal/analysis
MH  - *Drug Resistance, Fungal/genetics
MH  - Female
MH  - Fluconazole/*pharmacology
MH  - Genetic Heterogeneity
MH  - Genotype
MH  - Hematologic Neoplasms/drug therapy
MH  - Humans
MH  - Male
MH  - Microbial Sensitivity Tests
MH  - Middle Aged
MH  - Mouth Mucosa/*microbiology
MH  - Mycology/methods
MH  - Neoplasms/*drug therapy
MH  - Young Adult
PMC - PMC3475586
EDAT- 2010/08/10 06:00
MHDA- 2010/09/16 06:00
PMCR- 2009/09/01
CRDT- 2010/08/10 06:00
PHST- 2010/08/10 06:00 [entrez]
PHST- 2010/08/10 06:00 [pubmed]
PHST- 2010/09/16 06:00 [medline]
PHST- 2009/09/01 00:00 [pmc-release]
AID - ijos200922 [pii]
AID - 10.4248/IJOS.09020 [doi]
PST - ppublish
SO  - Int J Oral Sci. 2009 Sep;1(3):156-62. doi: 10.4248/IJOS.09020.