PMID- 20696152
OWN - NLM
STAT- MEDLINE
DCOM- 20110211
LR  - 20191210
IS  - 1096-0945 (Electronic)
IS  - 0014-4800 (Linking)
VI  - 90
IP  - 1
DP  - 2011 Feb
TI  - Evaluation of mild hyperhomocysteinemia during the development of atherosclerosis 
      in apolipoprotein E-deficient and normal mice.
PG  - 45-50
LID - 10.1016/j.yexmp.2010.07.008 [doi]
AB  - We focused on the effect of mild hyperhomocysteinemia (HHcy) on the development 
      of atherosclerosis, using apolipoprotein E-deficient (apoE(-/-)) and normal mice. 
      Mice received diets enriched in methionine with low or high levels of folate, 
      B(12) and B(6) (diets B and C, respectively), and diet only with low levels of 
      folate, B(12) and B(6) (diets D), to induce mild HHcy. Normal mice fed on diets 
      B, C and D presented mild HHcy, but they did not develop atherosclerotic lesions 
      after 24 weeks of diet. In addition, increased endoplasmic reticulum stress was 
      present in normal mice fed on diet B, compared to others groups. ApoE(-/-) mice 
      fed on diet B for 20 weeks presented the greatest atherosclerotic lesion area at 
      the aortic sinus than other groups. These results suggest that the methionine may 
      have a toxic effect on endothelium, and the B-vitamins addition on diet may have 
      a protective effect in the long term, despite the increase on homocysteine 
      levels. Mild HHcy accelerated the development of atherosclerosis in apoE(-/-) 
      mice, and supplementation with B-vitamins is important for prevention of vascular 
      disease, principally in the long term.
CI  - Copyright A(c) 2010 Elsevier Inc. All rights reserved.
FAU - Alessio, Ana C M
AU  - Alessio AC
AD  - Hematology-Hemotherapy Center, Faculty of Medical Sciences, State University of 
      Campinas (UNICAMP), Campinas, SP, Brazil. alessio@bol.com.br
FAU - Santos, Celio X C
AU  - Santos CX
FAU - Debbas, Victor
AU  - Debbas V
FAU - Oliveira, Laurione C
AU  - Oliveira LC
FAU - Haddad, Renato
AU  - Haddad R
FAU - Annichino-Bizzacchi, Joyce M
AU  - Annichino-Bizzacchi JM
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100806
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
RN  - 0 (Apolipoproteins E)
RN  - 935E97BOY8 (Folic Acid)
RN  - AE28F7PNPL (Methionine)
SB  - IM
MH  - Animals
MH  - Apolipoproteins E/*deficiency/genetics
MH  - Atherosclerosis/*complications/pathology/prevention & control
MH  - Diet
MH  - Endoplasmic Reticulum/drug effects/pathology
MH  - Folic Acid/pharmacology
MH  - Hyperhomocysteinemia/chemically induced/*complications/physiopathology
MH  - Male
MH  - Methionine/pharmacology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Time Factors
EDAT- 2010/08/11 06:00
MHDA- 2011/02/12 06:00
CRDT- 2010/08/11 06:00
PHST- 2010/01/18 00:00 [received]
PHST- 2010/07/29 00:00 [revised]
PHST- 2010/07/29 00:00 [accepted]
PHST- 2010/08/11 06:00 [entrez]
PHST- 2010/08/11 06:00 [pubmed]
PHST- 2011/02/12 06:00 [medline]
AID - S0014-4800(10)00105-X [pii]
AID - 10.1016/j.yexmp.2010.07.008 [doi]
PST - ppublish
SO  - Exp Mol Pathol. 2011 Feb;90(1):45-50. doi: 10.1016/j.yexmp.2010.07.008. Epub 2010 
      Aug 6.