PMID- 20698744
OWN - NLM
STAT- MEDLINE
DCOM- 20110112
LR  - 20101201
IS  - 1362-3095 (Electronic)
IS  - 0955-3002 (Linking)
VI  - 86
IP  - 12
DP  - 2010 Dec
TI  - Purple sweet potato pigments protect murine thymocytes from (6)(0)Co gamma-ray-induced 
      mitochondria-mediated apoptosis.
PG  - 1061-9
LID - 10.3109/09553002.2010.501840 [doi]
AB  - PURPOSE: Purple sweet potato (PSP) pigments have been widely accepted as 
      antioxidants but their radioprotective effect still remains unclear. In this 
      study we investigated the effect of PSP pigments on (6)(0)Co gamma-ray-induced 
      mitochondria-mediated apoptosis in murine thymocytes. MATERIALS AND METHODS: The 
      murine thymocytes were pretreated by PSP pigments before exposure to 4 Gy (6)(0)Co 
      gamma-rays. Flow cytometry analysis was used to measure apoptotic cells and 
      mitochondrial membrane potential. Reactive oxygen species (ROS) were detected 
      using 2',7',-dichlorofluorescein diacetate (DCFH-DA) probe and the activity of 
      antioxidant enzymes was tested by biochemical assay after irradiation. Cytochrome 
      c, caspase-3 and poly ADP-ribose polymerase (PARP) were measured by Western 
      blotting. RESULTS: After treatment with PSP pigments and exposure to 4 Gy 
      radiation the apoptosis of thymocytes was reduced and the mitochondrial 
      transmembrane potential was maintained compared to control cells. In the presence 
      of PSP pigments, ROS were reduced and the activities of glutathione peroxidase 
      (GSH-px) and superoxide dismutase (SOD) were protected and in some cases 
      increased. All the pro-apoptotic proteins (cytochrome oxidase, caspase 3 and 
      PARP) decreased in PSP pigments pretreated thymocytes compared to irradiated 
      cells in the absence of PSP pigments. CONCLUSIONS: Pre-treatment with PSP 
      pigments significantly inhibited (6)(0)Co gamma-ray-induced mitochondria-mediated 
      apoptosis. This radioprotective effect might be related to ROS scavenging, the 
      enhancement of the activity of antioxidant enzymes, the maintenance of 
      mitochondrial transmembrane potential, and the sequential inhibition of 
      cytochrome c release and downstream caspase and PARP cleavage.
FAU - Xie, Jing
AU  - Xie J
AD  - Department of Pharmacochemistry, Ocean University of China, Qingdao, PR China.
FAU - Han, Yan-Tao
AU  - Han YT
FAU - Wang, Chun-Bo
AU  - Wang CB
FAU - Yu, Wen-Gong
AU  - Yu WG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100810
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - 0 (Cobalt Radioisotopes)
RN  - 0 (Pigments, Biological)
RN  - 0 (Radiation-Protective Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - 9007-43-6 (Cytochromes c)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
RN  - EC 3.4.22.- (Casp3 protein, mouse)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects/radiation effects
MH  - Caspase 3/metabolism
MH  - Cell Survival/drug effects/radiation effects
MH  - Cells, Cultured
MH  - Cobalt Radioisotopes/toxicity
MH  - Cytochromes c/metabolism
MH  - Gamma Rays/adverse effects
MH  - Glutathione Peroxidase/metabolism
MH  - Ipomoea batatas
MH  - Membrane Potential, Mitochondrial/drug effects/radiation effects
MH  - Mice
MH  - Mitochondria/drug effects/metabolism/radiation effects
MH  - Pigments, Biological/*pharmacology
MH  - Poly(ADP-ribose) Polymerases/metabolism
MH  - Radiation-Protective Agents/*pharmacology
MH  - Reactive Oxygen Species/metabolism
MH  - Superoxide Dismutase/metabolism
MH  - T-Lymphocytes/*drug effects/metabolism/pathology/*radiation effects
EDAT- 2010/08/12 06:00
MHDA- 2011/01/13 06:00
CRDT- 2010/08/12 06:00
PHST- 2010/08/12 06:00 [entrez]
PHST- 2010/08/12 06:00 [pubmed]
PHST- 2011/01/13 06:00 [medline]
AID - 10.3109/09553002.2010.501840 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 2010 Dec;86(12):1061-9. doi: 10.3109/09553002.2010.501840. 
      Epub 2010 Aug 10.