PMID- 20699442
OWN - NLM
STAT- MEDLINE
DCOM- 20110106
LR  - 20210206
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 116
IP  - 23
DP  - 2010 Dec 2
TI  - Dendritic cells modulate platelet activity in IVIg-mediated amelioration of ITP 
      in mice.
PG  - 5002-9
LID - 10.1182/blood-2010-03-275123 [doi]
AB  - Intravenous immunoglobulin (IVIg) is an effective treatment against immune 
      thrombocytopenia (ITP). Previous studies suggested that IVIg exerts this 
      ameliorative role through 2 different leukocyte subsets. Dendritic cells (DCs) 
      modulate the immunosuppression in an adoptive cell transfer model, and phagocytes 
      up-regulate their inhibitory IgG Fc receptors (FcgammaR)IIB expression and thereby 
      ameliorate the inflammatory response and platelet clearance. However, whether or 
      not regulatory mechanisms exist among DCs, phagocytes, and platelets is still 
      largely unknown. In this study we present findings that IVIg-primed splenic 
      CD11c(+) DCs (IVIg-DCs) primarily mediate their anti-inflammatory effects at the 
      level of the platelet rather than the phagocyte. IVIg-DCs did not ameliorate ITP 
      in Fcgr2b(-/-), Fcgr3(-/-), nor P-Selp(-/-) mice, implicating the potential 
      involvement of these pathways in IVIg action. As platelets are a component of DC 
      regulatory circuits, these findings may suggest an alternative perspective for 
      the use of IVIg treatment.
FAU - Huang, Hsuan-Shun
AU  - Huang HS
AD  - Institute of Medical Sciences, Tzu-Chi University, Hualien, Taiwan, Republic of 
      China.
FAU - Sun, Der-Shan
AU  - Sun DS
FAU - Lien, Te-Sheng
AU  - Lien TS
FAU - Chang, Hsin-Hou
AU  - Chang HH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100810
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Fcgr2b protein, mouse)
RN  - 0 (Fcgr3 protein, mouse)
RN  - 0 (Immunoglobulins, Intravenous)
RN  - 0 (Receptors, IgG)
RN  - 0 (Selenoprotein P)
SB  - IM
CIN - Blood. 2010 Dec 2;116(23):4740-1. PMID: 21127183
MH  - Animals
MH  - Blood Platelets/drug effects/*immunology/metabolism
MH  - Cell Separation
MH  - Dendritic Cells/drug effects/*immunology/metabolism
MH  - Flow Cytometry
MH  - Immunoglobulins, Intravenous/immunology/*pharmacology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Purpura, Thrombocytopenic, Idiopathic/drug therapy/*immunology/metabolism
MH  - Receptors, IgG/immunology/metabolism
MH  - Selenoprotein P/immunology/metabolism
EDAT- 2010/08/12 06:00
MHDA- 2011/01/07 06:00
CRDT- 2010/08/12 06:00
PHST- 2010/08/12 06:00 [entrez]
PHST- 2010/08/12 06:00 [pubmed]
PHST- 2011/01/07 06:00 [medline]
AID - S0006-4971(20)60264-6 [pii]
AID - 10.1182/blood-2010-03-275123 [doi]
PST - ppublish
SO  - Blood. 2010 Dec 2;116(23):5002-9. doi: 10.1182/blood-2010-03-275123. Epub 2010 
      Aug 10.