PMID- 20701064
OWN - NLM
STAT- MEDLINE
DCOM- 20100826
LR  - 20221207
IS  - 0884-6812 (Linking)
VI  - 32
IP  - 3
DP  - 2010 Jun
TI  - Assessment of fluorescence in situ hybridization and hybrid capture 2 analyses of 
      cervical cytology specimens diagnosed as low grade squamous intraepithelial 
      lesion for the detection of high grade cervical intraepithelial neoplasia.
PG  - 121-30
AB  - OBJECTIVE: To assess Hybrid Capture 2 (HC2) and fluorescence in situ 
      hybridization (FISH) for the detection of cervical intraepithelial neoplasia 2 or 
      worse (CIN 2+) in patients with a cytologic diagnosis of low grade squamous 
      intraepithelial lesion (LSIL). STUDY DESIGN: Residual samples from 115 
      LSIL-diagnosed cervical cytology specimens were evaluated by high-risk human 
      papillomavirus (HR-HPV) HC2 testing and FISH using biotin-labeled probes to 
      HR-HPV and chromosomal probes to 3q26 (TERC) and 8q24 (CMYC). A cervical biopsy 
      diagnosis of CIN 2+ was considered as evidence of high grade disease. RESULTS: 
      The positive and negative predictive values of HC2 and FISH for detecting 
      patients with CIN 2+ were 32% vs. 37% and 100% vs. 93%, respectively. The 
      sensitivities of HC2 and FISH for CIN 2+ were not significantly different (100% 
      vs. 90%, p = 0.25), while the specificity of HC2 was significantly lower than 
      that of FISH (28% vs. 48%, p=0.003). FISH diagnosed fewer specimens as positive 
      as compared to HC2 (62% vs. 79%). CONCLUSION: These preliminary data suggest that 
      FISH testing may be useful for determining which patients with LSIL are most 
      likely to have CIN 2+ on clinical follow-up.
FAU - Voss, Jesse S
AU  - Voss JS
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic and Foundation, 
      Rochester, Minnesota 55905, USA.
FAU - Kipp, Benjamin R
AU  - Kipp BR
FAU - Campion, Michael B
AU  - Campion MB
FAU - Sokolova, Irina A
AU  - Sokolova IA
FAU - Henry, Michael R
AU  - Henry MR
FAU - Halling, Kevin C
AU  - Halling KC
FAU - Clayton, Amy C
AU  - Clayton AC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Anal Quant Cytol Histol
JT  - Analytical and quantitative cytology and histology
JID - 8506819
RN  - 0 (DNA, Neoplasm)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Aberrations
MH  - DNA, Neoplasm/analysis
MH  - DNA, Viral/analysis
MH  - False Positive Reactions
MH  - Feasibility Studies
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Middle Aged
MH  - Papillomaviridae/genetics/isolation & purification
MH  - Papillomavirus Infections/*diagnosis/virology
MH  - Predictive Value of Tests
MH  - ROC Curve
MH  - Uterine Cervical Neoplasms/*diagnosis/virology
MH  - Vaginal Smears
MH  - Young Adult
MH  - Uterine Cervical Dysplasia/*diagnosis/virology
EDAT- 2010/08/13 06:00
MHDA- 2010/08/27 06:00
CRDT- 2010/08/13 06:00
PHST- 2010/08/13 06:00 [entrez]
PHST- 2010/08/13 06:00 [pubmed]
PHST- 2010/08/27 06:00 [medline]
PST - ppublish
SO  - Anal Quant Cytol Histol. 2010 Jun;32(3):121-30.