PMID- 20701083
OWN - NLM
STAT- MEDLINE
DCOM- 20100824
LR  - 20161020
IS  - 0884-6812 (Linking)
VI  - 32
IP  - 1
DP  - 2010 Feb
TI  - A study of bone marrow neoangiogenesis in chronic lymphocytic leukemia patients.
PG  - 11-23
AB  - OBJECTIVE: To use a purely geometric data-unravelling method to identify, without 
      a priori, direct links of neoangiogenesis with known clinicobiologic variables in 
      23 untreated chronic lymphocytic leukemia (CLL) patients at the time of marrow 
      sampling. STUDY DESIGN: We measured neovascular surface density (CD34 endothelial 
      marker) in the 10 most labeled fields (hVA), cellularity (CA) and the percentage 
      of avascular fields (AVF) in bone marrow biopsy specimens from 34 patients with 
      CLL. Vascular endothelial growth factor (VEGF) was assayed in thawed serum 
      samples from 8 of the 23 untreated patients (12 Rai stage 0, 6 Rai stage I-II, 5 
      Binet stage B-C). RESULTS: Neoangiogenesis hVA was lower in the 12 stage O 
      patients (p < 0.039) and in the 6 patients with nodular interstitial mixed 
      infiltration (p = 0.058). The link between hVA and serum VEGF (sVEGF) was 
      negative in 8 patients (R = -0.49, -0.70 for a given age). Age was linked to 
      moderate interstitial infiltration (p < 0.02), AVF (R = 0.37) and lower sVEGF 
      levels (R = -0.676). The blood platelet count showed numerous correlations, 
      including links with AVF (R = 0.40), low hVA (R = -0.38), female sex (p = 0.068), 
      absence of splenomegaly (p < 0.001), mixed-type infiltration (p < 0.01) and sVEGF 
      (R = 0.38, 0.61 for a given age). CONCLUSION: The links revealed by the 
      iconography of correlations were described statistically. Surprisingly, negative 
      links of hVA with the sVEGF level, 2 known prognostic factors, on the one hand, 
      and with the blood platelet count, on the other hand, were found. The direct link 
      between disease progression and the amount of avascular hematopoietic tissue 
      (NS), not previously described, will require further study. Age should be taken 
      into account.
FAU - Lesty, Claude
AU  - Lesty C
AD  - Laboratory of Biologic Hematology, Hopital de la Pitie-Salpetriere, Paris, 
      France. claude.lesty@upmc.fr
FAU - Baudet, Sylvie
AU  - Baudet S
FAU - Charlotte, Frederic
AU  - Charlotte F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anal Quant Cytol Histol
JT  - Analytical and quantitative cytology and histology
JID - 8506819
RN  - 0 (Antigens, CD34)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Antigens, CD34/metabolism
MH  - Biopsy
MH  - Blood Vessels/metabolism/pathology
MH  - Bone Marrow/*blood supply
MH  - Disease Progression
MH  - Female
MH  - Hematologic Tests
MH  - Humans
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*etiology/pathology
MH  - Male
MH  - Middle Aged
MH  - Neovascularization, Pathologic/metabolism/*pathology
MH  - Vascular Endothelial Growth Factor A/metabolism
EDAT- 2010/08/13 06:00
MHDA- 2010/08/25 06:00
CRDT- 2010/08/13 06:00
PHST- 2010/08/13 06:00 [entrez]
PHST- 2010/08/13 06:00 [pubmed]
PHST- 2010/08/25 06:00 [medline]
PST - ppublish
SO  - Anal Quant Cytol Histol. 2010 Feb;32(1):11-23.