PMID- 20701940
OWN - NLM
STAT- MEDLINE
DCOM- 20101005
LR  - 20100913
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 406
IP  - 2
DP  - 2010 Oct 25
TI  - Porcine reproductive and respiratory syndrome virus non-structural protein 1 
      suppresses tumor necrosis factor-alpha promoter activation by inhibiting NF-kappaB 
      and Sp1.
PG  - 270-9
LID - 10.1016/j.virol.2010.07.016 [doi]
AB  - The objective of this study was to identify porcine reproductive and respiratory 
      syndrome virus (PRRSV)-encoded proteins that are responsible for the inhibition 
      of TNF-alpha expression and the mechanism(s) involved in this phenomenon. Using a 
      TNF-alpha promoter reporter system, the non-structural protein 1 (Nsp1) was found to 
      strongly suppress the TNF-alpha promoter activity. Such inhibition takes place 
      especially at the promoter's proximal region. Both Nsp1alpha and Nsp1beta, the two 
      proteolytic fragments of Nsp1, were shown to be involved in TNF-alpha promoter 
      suppression. Furthermore, using reporter plasmids specific for transcription 
      factors (TFs) that bind to TNF-alpha promoter, Nsp1alpha and Nsp1beta were demonstrated to 
      inhibit the activity of the TFs that bind CRE-kappaB(3) and Sp1 elements 
      respectively. Subsequent analyses showed that Nsp1alpha moderately inhibits NF-kappaB 
      activation and that Nsp1beta completely abrogates the Sp1 transactivation. These 
      findings reveal one of the important mechanisms underlying the innate immune 
      evasion by PRRSV during infection.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Subramaniam, Sakthivel
AU  - Subramaniam S
AD  - School of Veterinary Medicine & Biomedical Sciences, University of 
      Nebraska-Lincoln, NE 68583, USA.
FAU - Kwon, Byungjoon
AU  - Kwon B
FAU - Beura, Lalit K
AU  - Beura LK
FAU - Kuszynski, Charles A
AU  - Kuszynski CA
FAU - Pattnaik, Asit K
AU  - Pattnaik AK
FAU - Osorio, Fernando A
AU  - Osorio FA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100811
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (NF-kappa B)
RN  - 0 (Sp1 Transcription Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - *Down-Regulation
MH  - Mice
MH  - Molecular Sequence Data
MH  - NF-kappa B/genetics/*metabolism
MH  - Porcine Reproductive and Respiratory Syndrome/*genetics/metabolism
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*metabolism
MH  - *Promoter Regions, Genetic
MH  - Protein Binding
MH  - Sp1 Transcription Factor/genetics/*metabolism
MH  - Swine
MH  - Transcriptional Activation
MH  - Tumor Necrosis Factor-alpha/*genetics/metabolism
MH  - Viral Nonstructural Proteins/genetics/*metabolism
EDAT- 2010/08/13 06:00
MHDA- 2010/10/06 06:00
CRDT- 2010/08/13 06:00
PHST- 2010/06/04 00:00 [received]
PHST- 2010/07/10 00:00 [accepted]
PHST- 2010/08/13 06:00 [entrez]
PHST- 2010/08/13 06:00 [pubmed]
PHST- 2010/10/06 06:00 [medline]
AID - S0042-6822(10)00456-3 [pii]
AID - 10.1016/j.virol.2010.07.016 [doi]
PST - ppublish
SO  - Virology. 2010 Oct 25;406(2):270-9. doi: 10.1016/j.virol.2010.07.016. Epub 2010 
      Aug 11.