PMID- 20705001
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20100813
IS  - 1944-7930 (Electronic)
IS  - 1539-6509 (Linking)
VI  - 4
IP  - 21
DP  - 2004 Apr
TI  - High resolution definition of chromosome abnormalities with probes designed from 
      genome sequences.
PG  - 99-101
AB  - Extract: Numerical and structural chromosome abnormalities are a common cause of 
      inherited and acquired diseases in humans. Cytogenetic detection of genomic 
      imbalances and rearrangements is standard diagnostic practice, and is used both 
      prognostically and for treatment stratification, especially for neoplastic 
      disorders. Abnormalities are recognized by chromosome banding and by fluorescence 
      in situ hybridization (FISH). FISH permits examination of specific DNA sequences 
      within single or multiple chromosome bands on a metaphase cell or within an 
      interphase cell. Locus-specific FISH probes have traditionally been composed of 
      recombinant DNA segments that span large chromosomal targets of hundreds of 
      thousands of base pairs, about an order of magnitude smaller than the length of a 
      typical chromosomal band. These probes, which contain either repetitive 
      sequences, single copy sequences or combinations of both, have been developed to 
      hybridize to a wide spectrum of chromosomal targets -- encompassing a single gene 
      to an entire chromosome. Commercially available clones are generally useful for 
      detecting more common abnormalities, whereas, detection or characterization of 
      rare chromosomal abnormalities by FISH has relied on clones obtained from 
      research laboratories.
FAU - Knoll, Joan H M
AU  - Knoll JH
AD  - Laboratories of Human Molecular Genetics and Genomic Disorders, Children's Mercy 
      Hospitals and Clinics, University of Missouri, Kansas City, Missouri 64108, USA.
FAU - Rogan, Peter K
AU  - Rogan PK
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Discov Med
JT  - Discovery medicine
JID - 101250006
EDAT- 2004/04/01 00:00
MHDA- 2004/04/01 00:01
CRDT- 2010/08/14 06:00
PHST- 2010/08/14 06:00 [entrez]
PHST- 2004/04/01 00:00 [pubmed]
PHST- 2004/04/01 00:01 [medline]
PST - ppublish
SO  - Discov Med. 2004 Apr;4(21):99-101.