PMID- 20707866
OWN - NLM
STAT- MEDLINE
DCOM- 20110204
LR  - 20220331
IS  - 1474-9726 (Electronic)
IS  - 1474-9718 (Linking)
VI  - 9
IP  - 5
DP  - 2010 Oct
TI  - Modulation of white adipose tissue proteome by aging and calorie restriction.
PG  - 882-94
LID - 10.1111/j.1474-9726.2010.00613.x [doi]
AB  - Aging is associated with an accrual of body fat, progressive development of 
      insulin resistance and other obesity comorbidities that contribute to decrease 
      life span. Caloric restriction (CR), which primarily affects energy stores in 
      adipose tissue, is known to extend life span and retard the aging process in 
      animal models. In this study, a proteomic approach combining 2-DE and MS was used 
      to identify proteins modulated by aging and CR in rat white adipose tissue 
      proteome. Proteomic analysis revealed 133 differentially expressed spots, 57 of 
      which were unambiguously identified by MS. Although CR opposed part of the 
      age-associated protein expression patterns, many effects of CR were on proteins 
      unaltered by age, suggesting that the effects of CR on adipose tissue are only 
      weakly related to those of aging. Particularly, CR and aging altered glucose, 
      intermediate and lipid metabolism, with CR enhancing the expression of enzymes 
      involved in oxalacetate and NADPH production, lipid biosynthesis and lipolysis. 
      Consistently, insulin-beta and beta3-adrenergic receptors were also increased by CR, 
      which denotes improved sensitivity to lipogenic/lipolytic stimuli. Other 
      beneficial outcomes of CR were an improvement in oxidative stress, preventing the 
      age-associated decrease in several antioxidant enzymes. Proteins involved in 
      cytoskeleton, iron storage, energy metabolism and several proteins with novel or 
      unknown functions in adipose tissue were also modulated by age and/or CR. Such 
      orchestrated changes in expression of multiple proteins provide insights into the 
      mechanism underlying CR effects, ultimately allowing the discovery of new markers 
      of aging and targets for the development of CR-mimetics.
CI  - (c) 2010 The Authors Aging Cell (c) 2010 Blackwell Publishing Ltd/Anatomical Society 
      of Great Britain and Ireland.
FAU - Valle, Adamo
AU  - Valle A
AD  - Institut Universitari d'Investigacio en Ciencies de la Salut, Universitat de les 
      Illes Balears, Spain. adamo.valle@uib.es
FAU - Sastre-Serra, Jordi
AU  - Sastre-Serra J
FAU - Roca, Pilar
AU  - Roca P
FAU - Oliver, Jordi
AU  - Oliver J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Aging Cell
JT  - Aging cell
JID - 101130839
RN  - 0 (Proteome)
SB  - IM
MH  - Adipose Tissue, White/*metabolism
MH  - Aging/*physiology
MH  - Animals
MH  - Body Weight
MH  - *Caloric Restriction
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Male
MH  - Mass Spectrometry
MH  - Proteome/isolation & purification/*metabolism
MH  - Proteomics
MH  - Rats
MH  - Rats, Wistar
EDAT- 2010/08/17 06:00
MHDA- 2011/02/05 06:00
CRDT- 2010/08/17 06:00
PHST- 2010/08/17 06:00 [entrez]
PHST- 2010/08/17 06:00 [pubmed]
PHST- 2011/02/05 06:00 [medline]
AID - ACE613 [pii]
AID - 10.1111/j.1474-9726.2010.00613.x [doi]
PST - ppublish
SO  - Aging Cell. 2010 Oct;9(5):882-94. doi: 10.1111/j.1474-9726.2010.00613.x.