PMID- 20708068
OWN - NLM
STAT- MEDLINE
DCOM- 20101228
LR  - 20161125
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 484
IP  - 1
DP  - 2010 Oct 22
TI  - Modulators of BK and SK channels alter electrical activity in vitro in single 
      vasopressin neurons isolated from the rat supraoptic nucleus.
PG  - 26-9
LID - 10.1016/j.neulet.2010.08.010 [doi]
AB  - Release of arginine vasopressin (AVP) from magnocellular neurosecretory cells 
      (MNCs) of the supraoptic nucleus (SON) is controlled by the electrical activities 
      of the MNCs. Ca(2+)-activated K(+) channels, such as the BK and SK channels, are 
      K(+)-selective ion channels that are activated in response to increased 
      intracellular calcium concentrations. Intrinsic affinities for Ca(2+) permit 
      these channels to exert a negative feedback effect on cellular excitability. In 
      the present study, we used the whole-cell patch-clamp technique to examine the 
      effects of BK or SK channel modulators on neuronal activity in single isolated 
      rat SON MNCs that express an AVP-enhanced green fluorescent protein (eGFP) 
      transgene. Application of BK or SK channel activators abolished the action 
      potentials and induced hyperpolarization. In contrast, the number of action 
      potentials was significantly increased after application of BK or SK channel 
      blockers. Our results suggest that BK and SK channels in AVP neurons may play a 
      role in the regulatory mechanisms of neural activity.
CI  - Copyright 2010 Elsevier Ireland Ltd. All rights reserved.
FAU - Ohbuchi, Toyoaki
AU  - Ohbuchi T
AD  - Department of Physiology, School of Medicine, University of Occupational and 
      Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
FAU - Yokoyama, Toru
AU  - Yokoyama T
FAU - Saito, Takeshi
AU  - Saito T
FAU - Suzuki, Hitoshi
AU  - Suzuki H
FAU - Fujihara, Hiroaki
AU  - Fujihara H
FAU - Katoh, Akiko
AU  - Katoh A
FAU - Otsubo, Hiroki
AU  - Otsubo H
FAU - Ishikura, Toru
AU  - Ishikura T
FAU - Suzuki, Hideaki
AU  - Suzuki H
FAU - Ueta, Yoichi
AU  - Ueta Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100811
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Large-Conductance Calcium-Activated Potassium Channels)
RN  - 0 (Peptides)
RN  - 0 (Small-Conductance Calcium-Activated Potassium Channels)
RN  - 11000-17-2 (Vasopressins)
RN  - 115422-61-2 (Charybdotoxin)
RN  - 24345-16-2 (Apamin)
RN  - 773HER9B6T (iberiotoxin)
SB  - IM
MH  - Action Potentials/drug effects/physiology
MH  - Animals
MH  - Apamin/pharmacology
MH  - Charybdotoxin/pharmacology
MH  - Large-Conductance Calcium-Activated Potassium Channels/*physiology
MH  - Male
MH  - Membrane Potentials/drug effects/physiology
MH  - Neurons/*drug effects/physiology
MH  - Patch-Clamp Techniques
MH  - Peptides/pharmacology
MH  - Rats
MH  - Rats, Transgenic
MH  - Small-Conductance Calcium-Activated Potassium Channels/*physiology
MH  - Supraoptic Nucleus/cytology/*drug effects/physiology
MH  - Vasopressins/*metabolism
EDAT- 2010/08/17 06:00
MHDA- 2010/12/29 06:00
CRDT- 2010/08/17 06:00
PHST- 2010/05/23 00:00 [received]
PHST- 2010/08/03 00:00 [revised]
PHST- 2010/08/04 00:00 [accepted]
PHST- 2010/08/17 06:00 [entrez]
PHST- 2010/08/17 06:00 [pubmed]
PHST- 2010/12/29 06:00 [medline]
AID - S0304-3940(10)01044-X [pii]
AID - 10.1016/j.neulet.2010.08.010 [doi]
PST - ppublish
SO  - Neurosci Lett. 2010 Oct 22;484(1):26-9. doi: 10.1016/j.neulet.2010.08.010. Epub 
      2010 Aug 11.