PMID- 20708412
OWN - NLM
STAT- MEDLINE
DCOM- 20120105
LR  - 20151119
IS  - 1879-1336 (Electronic)
IS  - 1054-8807 (Linking)
VI  - 20
IP  - 5
DP  - 2011 Sep-Oct
TI  - Immunohistochemical properties in the patients with Buerger's disease--possible 
      role of plasminogen activator inhibitor-1 for preservation of vessel wall 
      architecture.
PG  - 266-71
LID - 10.1016/j.carpath.2010.06.012 [doi]
AB  - BACKGROUND: The architecture of the arterial wall affected with Buerger's disease 
      has been known to be preserved in all three layers, while the one affected by 
      arteriosclerosis obliterans (ASO) is degenerated and destroyed. We analyzed 
      affected arteries with immunohistochemical methods to clarify the differences 
      between Buerger's disease and ASO. MATERIALS AND METHODS: Crural arteries 
      obtained from 13 patients with Buerger's disease and 6 patients with ASO at our 
      institute were studied. In addition, we examined seven specimens from six 
      patients who were thought to be normal (without Buerger's disease or ASO) as 
      negative control. Immunohistochemical studies were performed on paraffin-embedded 
      tissues. The primary antibodies were urokinase-type plasminogen activator (uPA) 
      and matrix metalloproteinase-3 (MMP-3). Both are known to play an important role 
      of extracellular proteolysis and to activate each other. Additionally, 
      plasminogen activator inhibitor-1(PAI-1) was also examined. RESULTS: In Buerger's 
      disease, PAI-1 was well expressed along the internal elastic lamina. 
      Urokinase-type plasminogen activator and MMP-3 were slightly positive in intima 
      and media. In ASO, a slight amount of PAI-1 was recognized on vessel walls, and 
      both uPA and MMP-3 were strongly positive in media. In addition, in the control 
      group, PAI-1, uPA, and MMP-3 were well expressed in media. CONCLUSION: In 
      Buerger's disease, PAI-1 was strongly expressed around the internal elastic 
      lamina, while both uPA and MMP-3 were slightly recognized on vessel walls. These 
      findings could be one of the reasons the general architecture of vessel walls in 
      Buerger's disease is preserved.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Sato, Toshimitsu
AU  - Sato T
AD  - Division of Vascular Surgery, Department of Surgery, Nagoya University Graduate 
      School of Medicine, Japan. sato-toshimitsu@pref.gifu.lg.jp
FAU - Tamai, Hiroaki
AU  - Tamai H
FAU - Kobayashi, Masayoshi
AU  - Kobayashi M
FAU - Yamamoto, Kiyohito
AU  - Yamamoto K
FAU - Komori, Kimihiro
AU  - Komori K
LA  - eng
PT  - Journal Article
DEP - 20100812
PL  - United States
TA  - Cardiovasc Pathol
JT  - Cardiovascular pathology : the official journal of the Society for Cardiovascular 
      Pathology
JID - 9212060
RN  - 0 (Biomarkers)
RN  - 0 (Plasminogen Activator Inhibitor 1)
RN  - 0 (SERPINE1 protein, human)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adult
MH  - Arteries/metabolism/*pathology
MH  - Arteriosclerosis Obliterans/metabolism/pathology
MH  - Biomarkers/metabolism
MH  - Calcinosis/metabolism/pathology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Matrix Metalloproteinase 3/metabolism
MH  - Middle Aged
MH  - Plasminogen Activator Inhibitor 1/*metabolism
MH  - Thromboangiitis Obliterans/metabolism/*pathology
MH  - Urokinase-Type Plasminogen Activator/metabolism
EDAT- 2010/08/17 06:00
MHDA- 2012/01/06 06:00
CRDT- 2010/08/17 06:00
PHST- 2008/12/14 00:00 [received]
PHST- 2010/03/11 00:00 [revised]
PHST- 2010/06/29 00:00 [accepted]
PHST- 2010/08/17 06:00 [entrez]
PHST- 2010/08/17 06:00 [pubmed]
PHST- 2012/01/06 06:00 [medline]
AID - S1054-8807(10)00110-9 [pii]
AID - 10.1016/j.carpath.2010.06.012 [doi]
PST - ppublish
SO  - Cardiovasc Pathol. 2011 Sep-Oct;20(5):266-71. doi: 10.1016/j.carpath.2010.06.012. 
      Epub 2010 Aug 12.