PMID- 20709031
OWN - NLM
STAT- MEDLINE
DCOM- 20110118
LR  - 20181201
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1358
DP  - 2010 Oct 28
TI  - Age-dependent modifications in the mRNA levels of the rat excitatory amino acid 
      transporters (EAATs) at 48hour reperfusion following global ischemia.
PG  - 11-9
LID - 10.1016/j.brainres.2010.08.020 [doi]
AB  - This study reports the mRNA levels of some excitatory amino acid transporters 
      (EAATs) in response to ischemia-reperfusion (I/R) in rat hippocampus and cerebral 
      cortex. The study was performed in 3-month-old and 18-month-old animals to 
      analyze the possible role of age in the I/R response of these transporters. The 
      I/R resulted in a reduced transcription of both the neuronal EAAC1 (excitatory 
      amino acid carrier-1) and the neuronal and glial GLT-1 (glial glutamate 
      transporter 1), while the glial GLAST1a (l-glutamate/l-aspartate transporter 1a) 
      transcription increased following I/R. The changes observed were more striking in 
      3-month-old animals than in 18-month-old animals. We hypothesize that increases 
      in the GLAST1a mRNA levels following I/R insult can be explained by increases in 
      glial cells, while the GLT-1 response to I/R mirrors neuronal changes. GLAST1a 
      transcription increases in 3-month-old animals support the hypothesis that this 
      transporter would be the main mechanism for extracellular glutamate clearance 
      after I/R. Decreases in EAAC1 and GLT-1 mRNA levels would represent either 
      neuronal changes due to the delayed neuronal death or a putative protective 
      down-regulation of these transporters to decrease the amount of glutamate inside 
      the neurons, which would decrease their glutamate release. This study also 
      reports how the treatment with the anti-inflammatory agent meloxicam attenuates 
      the transcriptional response to I/R in 3-month-old rats and decreases the 
      survival of the I/R-injured animals.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Montori, Sheyla
AU  - Montori S
AD  - Area de Biologia Celular, Instituto de Biomedicina, Universidad de Leon, 24071 
      Leon, Spain.
FAU - Martinez-Villayandre, Beatriz
AU  - Martinez-Villayandre B
FAU - Dos-Anjos, Severiano
AU  - Dos-Anjos S
FAU - Llorente, Irene L
AU  - Llorente IL
FAU - Burgin, Taiana C
AU  - Burgin TC
FAU - Fernandez-Lopez, Arsenio
AU  - Fernandez-Lopez A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100813
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Excitatory Amino Acid Transporter 2)
RN  - 0 (Excitatory Amino Acid Transporter 3)
RN  - 0 (RNA, Messenger)
RN  - 0 (Slc1a1 protein, rat)
RN  - 0 (Thiazines)
RN  - 0 (Thiazoles)
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
RN  - VG2QF83CGL (Meloxicam)
SB  - IM
MH  - *Aging
MH  - Analysis of Variance
MH  - Animals
MH  - Anti-Inflammatory Agents/therapeutic use
MH  - Cerebral Cortex/drug effects/metabolism
MH  - Disease Models, Animal
MH  - Encephalitis/drug therapy/etiology
MH  - Excitatory Amino Acid Transporter 2/*genetics/metabolism
MH  - Excitatory Amino Acid Transporter 3/*genetics/metabolism
MH  - Hippocampus/drug effects/metabolism
MH  - Ischemia/complications/*metabolism/pathology
MH  - Male
MH  - Meloxicam
MH  - Phosphopyruvate Hydratase/genetics/metabolism
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Reperfusion
MH  - Thiazines/therapeutic use
MH  - Thiazoles/therapeutic use
EDAT- 2010/08/17 06:00
MHDA- 2011/01/19 06:00
CRDT- 2010/08/17 06:00
PHST- 2010/02/12 00:00 [received]
PHST- 2010/08/06 00:00 [revised]
PHST- 2010/08/07 00:00 [accepted]
PHST- 2010/08/17 06:00 [entrez]
PHST- 2010/08/17 06:00 [pubmed]
PHST- 2011/01/19 06:00 [medline]
AID - S0006-8993(10)01767-1 [pii]
AID - 10.1016/j.brainres.2010.08.020 [doi]
PST - ppublish
SO  - Brain Res. 2010 Oct 28;1358:11-9. doi: 10.1016/j.brainres.2010.08.020. Epub 2010 
      Aug 13.