PMID- 20712117
OWN - NLM
STAT- MEDLINE
DCOM- 20120105
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 28
IP  - 4
DP  - 2010 Apr
TI  - [Determination of azaspiracid-1 in shellfishes by liquid chromatography with 
      tandem mass spectrometry].
PG  - 363-7
AB  - A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the 
      determination of azaspiracid-1 (AZA1) in shellfishes was described. After being 
      extracted using methanol and water (80:20, v/v), the extract was cleaned-up by 
      solid phase extraction (SPE) of MAX column, then determined by using a 
      reversed-phase high performance liquid chromatography (HPLC) isocratic program 
      coupled with tandem mass spectrometry in selected reaction monitoring mode (SRM). 
      And the extract was eluted with acetonitrile-water (80:20, v/v) on an Atlantis 
      dC18 column (150 mm x 4.6 mm, 5.0 microm) with mobile phase containing 50 mmol/L 
      formic acid and 2 mmol/L ammonium formate. The detection limit was 11.00 pg/g. 
      The calibration curve was linear (R2 = 0.998 1) in the range of 48.85-2 442 ng/L. 
      The average recoveries of the shellfish tissue extract at three spiked levels 
      (36.64, 73.27, 146.54 pg/g) were from 75.8% to 82.5% (n = 6). The relative 
      standard derivations (RSDs) were less than 10%. The 112 shellfish samples from 
      the local markets of Dalian, Qingdao, Guangzhou were detected by the method, and 
      AZA1 was detected in some samples from Dalian and Guangzhou. The results showed 
      that the method is simple, rapid, sensitive and suitable for the detection of 
      AZA1 in shellfishes.
FAU - Yao, Jianhua
AU  - Yao J
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao 266071, China.
FAU - Tan, Zhijun
AU  - Tan Z
FAU - Zhou, Deqing
AU  - Zhou D
FAU - Guo, Mengmeng
AU  - Guo M
FAU - Xing, Lihong
AU  - Xing L
FAU - Yang, Shouguo
AU  - Yang S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Marine Toxins)
RN  - 0 (Spiro Compounds)
RN  - 0 (azaspiracid)
SB  - IM
MH  - Animals
MH  - Bivalvia/chemistry
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Marine Toxins/*analysis
MH  - Sensitivity and Specificity
MH  - Shellfish/*analysis
MH  - Spiro Compounds/*analysis
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2010/08/18 06:00
MHDA- 2012/01/06 06:00
CRDT- 2010/08/18 06:00
PHST- 2010/08/18 06:00 [entrez]
PHST- 2010/08/18 06:00 [pubmed]
PHST- 2012/01/06 06:00 [medline]
AID - 10.3724/sp.j.1123.2010.00363 [doi]
PST - ppublish
SO  - Se Pu. 2010 Apr;28(4):363-7. doi: 10.3724/sp.j.1123.2010.00363.