PMID- 20712120
OWN - NLM
STAT- MEDLINE
DCOM- 20120105
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 28
IP  - 4
DP  - 2010 Apr
TI  - [Determination of trigonelline in Trigonella foenum-graecum L. by hydrophilic 
      interaction chromatography].
PG  - 379-82
AB  - A method of hydrophilic interaction chromatography (HILIC) was established for 
      the quantitative determination of trigonelline in Trigonella foenum-graecum L. 
      HILIC analysis was performed on a Waters Atlantis HILIC Silica column (150 mm x 
      2.1 mm, 3 microm). The mobile phase consisted of acetonitrile-ammonium acetate 
      (pH 4.4) (70:30, v/v), and the flow rate was 0. 4 mL/min. The detection 
      wavelength was set at 265 nm. The method has good linearity in the range of 
      2.50-100 mg/L for trigonelline (r = 0.999 6). The recoveries were on an average 
      of 102% by adding 29.2 mg/L and 43.8 mg/L with relative standard deviations 
      (RSDs) of 4.17% and 2.28% (n = 3), respectively. The results indicate that the 
      method is simple and rapid for the determination of strong polar trigonelline in 
      Trigonella foenum-graecum L. Furthermore, it significantly reduces the 
      equilibration time compared with ion-pair liquid chromatography (IPLC) recorded 
      in the Pharmacopoeia of China. This new method can be used as a valid method for 
      the quality control of Trigonella foenum-graecum L.
FAU - Zhuo, Rongjie
AU  - Zhuo R
AD  - Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, 
      China.
FAU - Wang, Li
AU  - Wang L
FAU - Wang, Longxing
AU  - Wang L
FAU - Xiao, Hongbin
AU  - Xiao H
FAU - Cai, Shaoqing
AU  - Cai S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Alkaloids)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 3NQ9N60I00 (trigonelline)
SB  - IM
MH  - Alkaloids/*analysis/chemistry
MH  - Chromatography/instrumentation/*methods
MH  - Drugs, Chinese Herbal/analysis
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Trigonella/*chemistry
EDAT- 2010/08/18 06:00
MHDA- 2012/01/06 06:00
CRDT- 2010/08/18 06:00
PHST- 2010/08/18 06:00 [entrez]
PHST- 2010/08/18 06:00 [pubmed]
PHST- 2012/01/06 06:00 [medline]
AID - 10.3724/sp.j.1123.2010.00379 [doi]
PST - ppublish
SO  - Se Pu. 2010 Apr;28(4):379-82. doi: 10.3724/sp.j.1123.2010.00379.