PMID- 20712122
OWN - NLM
STAT- MEDLINE
DCOM- 20120105
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 28
IP  - 4
DP  - 2010 Apr
TI  - [Determination of five hormone drug residues in fish tissue by high performance 
      liquid chromatography with gel permeation chromatographic clean-up].
PG  - 388-92
AB  - A method was developed for the determination of five hormone residues in fish 
      tissue by high performance liquid chromatography (HPLC) with gel permeation 
      chromatographic (GPC) clean-up. The sample was extracted with ethyl 
      acetate-methanol (8:2, v/v). The extract was cleaned-up on a Pharmadex LH-20 gel 
      permeation column (450 mm x 15 mm) and eluted with methanol-ethyl acetate-acetic 
      acid (800:200:2, v/v/v). The analysis was performed on an Agilent TC-C18 column 
      (250 mm x 4. 6 mm, 5 microm) using acetonitrile-water (45:55, v/v) as the mobile 
      phase at a flow rate of 1.2 mL/min and the detection wavelengths were set at 222 
      nm and 245 nm. All of the 5 hormone residues had good linear relationships (r > 
      0.999) in the range of 0.05-2.5 mg/L. The limits of detection (LOD) were from 10 
      to 24 microg/kg. The average recoveries for all the five hormone residues were 
      from 60.1% to 89.0%, with relative standard deviations (RSDs) from 2.0% to 7.4%. 
      The method is simple, rapid, and can be applied for the analysis of the five 
      hormone residues in fish tissue.
FAU - Xie, Weiping
AU  - Xie W
AD  - Department of Physical and Chemical Analysis, Quanzhou Center for Disease Control 
      and Prevention, Quanzhou 362000, China. xweiping2000@163.com
FAU - Ouyang, Yanling
AU  - Ouyang Y
FAU - Huang, Yingyu
AU  - Huang Y
FAU - Chen, Chunzhu
AU  - Chen C
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Gonadal Steroid Hormones)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Chromatography, Gel/*methods
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drug Residues/*analysis
MH  - Estradiol/analysis
MH  - Fish Products/*analysis
MH  - Gonadal Steroid Hormones/*analysis
EDAT- 2010/08/18 06:00
MHDA- 2012/01/06 06:00
CRDT- 2010/08/18 06:00
PHST- 2010/08/18 06:00 [entrez]
PHST- 2010/08/18 06:00 [pubmed]
PHST- 2012/01/06 06:00 [medline]
AID - 10.3724/sp.j.1123.2010.00388 [doi]
PST - ppublish
SO  - Se Pu. 2010 Apr;28(4):388-92. doi: 10.3724/sp.j.1123.2010.00388.