PMID- 20712451
OWN - NLM
STAT- MEDLINE
DCOM- 20101104
LR  - 20101118
IS  - 1748-295X (Electronic)
IS  - 1748-2941 (Linking)
VI  - 12
IP  - 3
DP  - 2010 Sep
TI  - Cell free DNA detected by a novel method in acute ST-elevation myocardial 
      infarction patients.
PG  - 109-11
LID - 10.3109/17482941.2010.513732 [doi]
AB  - BACKGROUND: High levels of circulating cell free DNA (CFD) have been associated 
      with poor prognosis in various diseases. Data pertaining to CFD in acute 
      myocardial infarction (MI) are scarce. The available data have been obtained by 
      either electrophoresis or polymerase chain reaction. We evaluated a novel method 
      for the detection of CFD in patients with ST elevation myocardial infarction 
      (STEMI) and examined its correlation with established markers of necrosis and 
      ventricular function. METHODS: Serum concentrations of CFD, troponin-T and 
      creatine kinase (CK) were measured simultaneously in 16 randomly selected acute 
      STEMI patients upon admission and at three more time points. 47 healthy subjects 
      served as a control group. CFD was quantified by a novel rapid fluorometric 
      assay. Ejection fraction (EF) was assessed by echocardiography. RESULTS: Peak CFD 
      levels were significantly higher in patients compared with controls (P = 0.001) 
      and correlated with peak levels of CK and troponin-T (R = 0.79, P <0.001); R = 
      0.65, P = 0.006, respectively). Peak CFD levels tended to be associated with 
      lower EF (P = 0.075). CONCLUSION: With this method, CFD levels correlated with 
      the levels of established markers of myocardial necrosis but not with EF. The 
      kinetic pattern of CFD release after STEMI and its prognostic value require 
      further investigation.
FAU - Shimony, Avi
AU  - Shimony A
AD  - Department of Cardiology, Soroka University Medical Center, Ben Gurion University 
      of the Negev, Beer Sheva, Israel. ashimony@bgu.ac.il;
FAU - Zahger, Doron
AU  - Zahger D
FAU - Gilutz, Harel
AU  - Gilutz H
FAU - Goldstein, Hagit
AU  - Goldstein H
FAU - Orlov, Gennady
AU  - Orlov G
FAU - Merkin, Miri
AU  - Merkin M
FAU - Shalev, Aryeh
AU  - Shalev A
FAU - Ilia, Reuben
AU  - Ilia R
FAU - Douvdevani, Amos
AU  - Douvdevani A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Acute Card Care
JT  - Acute cardiac care
JID - 101276603
RN  - 0 (Troponin T)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.3.2 (Creatine Kinase)
SB  - IM
MH  - Creatine Kinase/blood
MH  - *DNA/blood
MH  - Echocardiography
MH  - Electrocardiography
MH  - Fluorometry/*methods
MH  - Humans
MH  - Myocardial Infarction/blood/*diagnosis/physiopathology/therapy
MH  - Myocardium/metabolism/pathology
MH  - Necrosis/*genetics/metabolism
MH  - Prognosis
MH  - Stroke Volume
MH  - Troponin T/blood
EDAT- 2010/08/18 06:00
MHDA- 2010/11/05 06:00
CRDT- 2010/08/18 06:00
PHST- 2010/08/18 06:00 [entrez]
PHST- 2010/08/18 06:00 [pubmed]
PHST- 2010/11/05 06:00 [medline]
AID - 10.3109/17482941.2010.513732 [doi]
PST - ppublish
SO  - Acute Card Care. 2010 Sep;12(3):109-11. doi: 10.3109/17482941.2010.513732.