PMID- 20712487
OWN - NLM
STAT- MEDLINE
DCOM- 20110126
LR  - 20100817
IS  - 1557-8976 (Electronic)
IS  - 0882-8245 (Linking)
VI  - 23
IP  - 4
DP  - 2010 Aug
TI  - Interleukin-10 antisense oligodeoxynucleotide suppresses IL-10 expression and 
      effects on proinflammatory cytokine responses to porcine reproductive and 
      respiratory syndrome virus.
PG  - 425-35
LID - 10.1089/vim.2009.0066 [doi]
AB  - Upregulation of interleukin-10 (IL-10) expression has been suggested to be the 
      mechanism by which the porcine reproductive and respiratory syndrome virus 
      (PRRSV) suppresses the innate and adaptive immune response in infected pigs. In 
      this study we evaluated the potential of phosphorothioate-modified IL-10 
      antisense oligodeoxynucleotide specific to the translation initiation region of 
      porcine IL-10 mRNA (IL-10AS) in enhancing proinflammatory cytokine responses to 
      PRRSV. Naive peripheral blood mononuclear cells from eight PRRSV-seronegative 
      pigs were transfected with IL-10AS in vitro prior to PRRSV inoculation and 
      phorbol 12-myristate 13-acetate plus ionomycin or concanavalin A stimulation. The 
      effects of IL-10AS on mRNA expression of IL-10, interferon-gamma (IFN-gamma), 
      IFN-alpha, tumor necrosis factor-alpha (TNF-alpha), IL-2, and IL-4 were tested by 
      real-time PCR. The percentages of IFN-gamma-producing T-cell subsets were 
      determined by flow cytometry. Compared to the controls, the levels of IL-10 and 
      IL-2 mRNA were significantly reduced, while those of IFN-gamma mRNA were 
      increased, and TNF-alpha, IFN-alpha, and IL-4 mRNA were unchanged. An increase in 
      the percentage of the IFN-gamma+ population was also observed in lymphocytes and 
      CD8beta+ T cells. Our results suggest that IL-10AS has the potential to enhance 
      proinflammatory cytokine responses to PRRSV infection.
FAU - Charerntantanakul, Wasin
AU  - Charerntantanakul W
AD  - Department of Biology, Faculty of Science, Maejo University, Chiang Mai, 
      Thailand. wasin@mju.ac.th
FAU - Kasinrerk, Watchara
AU  - Kasinrerk W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
RN  - 0 (Oligodeoxyribonucleotides, Antisense)
RN  - 130068-27-8 (Interleukin-10)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - CD8-Positive T-Lymphocytes/immunology/metabolism
MH  - Cells, Cultured
MH  - Down-Regulation/immunology
MH  - Interferon-gamma/metabolism
MH  - Interleukin-10/*antagonists & inhibitors/genetics
MH  - Leukocytes, Mononuclear/immunology/metabolism
MH  - Oligodeoxyribonucleotides, Antisense/*genetics
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/metabolism
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Swine
MH  - Transfection
MH  - Up-Regulation/immunology
EDAT- 2010/08/18 06:00
MHDA- 2011/01/28 06:00
CRDT- 2010/08/18 06:00
PHST- 2010/08/18 06:00 [entrez]
PHST- 2010/08/18 06:00 [pubmed]
PHST- 2011/01/28 06:00 [medline]
AID - 10.1089/vim.2009.0066 [doi]
PST - ppublish
SO  - Viral Immunol. 2010 Aug;23(4):425-35. doi: 10.1089/vim.2009.0066.