PMID- 20712646
OWN - NLM
STAT- MEDLINE
DCOM- 20101216
LR  - 20191210
IS  - 1440-1827 (Electronic)
IS  - 1320-5463 (Linking)
VI  - 60
IP  - 9
DP  - 2010 Sep
TI  - Diagnostic utility of EWS break-apart fluorescence in situ hybridization in 
      distinguishing between non-cutaneous melanoma and clear cell sarcoma.
PG  - 608-13
LID - 10.1111/j.1440-1827.2010.02570.x [doi]
AB  - Clear cell sarcoma (CCS) is a rare soft tissue sarcoma with morphological 
      similarities to malignant melanoma (MM), but with a distinct genetic background 
      that includes the chromosomal translocation t(12;22)(q13;q12). Clear cell sarcoma 
      is often misdiagnosed as MM because of similarities in target locations and 
      immunophenotypes. Eighteen cases with MM in non-cutaneous sites were subjected to 
      fluorescence in situ hybridization (FISH) to assess EWS gene breakage. Tissue 
      microarrays were constructed using formalin-fixed, paraffin-embedded tissue and 
      the EWSR1 (22q12) dual-color, break-apart rearrangement probe (Vysis) was used. 
      Two patients were classified as CCS with EWS gene rearrangement, with a mean of 
      67.5% positive cells per sample according to break-apart FISH. The remaining 16 
      patients lacked break-apart signals of the EWS gene. The presence of type 1 (EWS 
      exon 8-ATF1 exon 4) fusion transcripts was confirmed in FISH-positive patients by 
      RT-PCR. Retrospective analysis revealed that the masses were located in the foot 
      and buttock, respectively. Morphologically, tumor cells were not typical for 
      those of CCS or MM. Break-apart FISH is an accurate and convenient method for 
      differentiating between MM and CCS. Molecular detection of EWS gene 
      rearrangement, either by break-apart FISH or RT-PCR, is mandatory in subjects 
      with melanotic tumors of soft tissue.
FAU - Song, Joon Seon
AU  - Song JS
AD  - Department of Pathology, University of Ulsan College of Medicine, Asan Medical 
      Center, Songpa-gu, Seoul, Korea.
FAU - Choi, Jene
AU  - Choi J
FAU - Kim, Ji Hun
AU  - Kim JH
FAU - Jang, Se Jin
AU  - Jang SJ
FAU - Cho, Kyung-Ja
AU  - Cho KJ
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - Australia
TA  - Pathol Int
JT  - Pathology international
JID - 9431380
RN  - 0 (RNA-Binding Protein EWS)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Diagnosis, Differential
MH  - Exons/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Melanoma/*diagnosis/genetics
MH  - Middle Aged
MH  - Oligonucleotide Array Sequence Analysis
MH  - RNA-Binding Protein EWS/*genetics
MH  - Retrospective Studies
MH  - Sarcoma, Clear Cell/*diagnosis/genetics
MH  - Soft Tissue Neoplasms/*diagnosis/genetics
MH  - Translocation, Genetic/genetics
EDAT- 2010/08/18 06:00
MHDA- 2010/12/17 06:00
CRDT- 2010/08/18 06:00
PHST- 2010/08/18 06:00 [entrez]
PHST- 2010/08/18 06:00 [pubmed]
PHST- 2010/12/17 06:00 [medline]
AID - PIN2570 [pii]
AID - 10.1111/j.1440-1827.2010.02570.x [doi]
PST - ppublish
SO  - Pathol Int. 2010 Sep;60(9):608-13. doi: 10.1111/j.1440-1827.2010.02570.x.