PMID- 20713172
OWN - NLM
STAT- MEDLINE
DCOM- 20110310
LR  - 20211020
IS  - 1769-714X (Electronic)
IS  - 1286-4579 (Print)
IS  - 1286-4579 (Linking)
VI  - 12
IP  - 14-15
DP  - 2010 Dec
TI  - Intracerebral vaccination suppresses the spread of rabies virus in the mouse 
      brain.
PG  - 1163-9
LID - 10.1016/j.micinf.2010.08.002 [doi]
AB  - To investigate the efficacy of intracerebral (IC) immunization in preventing 
      viral spread in the brain, we immunized mice with inactivated rabies virus via 
      the subcutaneous (SC) or IC route, followed by administration of a lethal dose of 
      rabies virus (challenge virus standard strain), directly into the brains of 
      immunized mice. Progressive paralytic neurological signs were observed in control 
      and 75% of SC immunized mice, whereas only 20% of IC immunized mice exhibited 
      symptoms. Neutralizing antibody titers in blood plasma were significantly 
      elevated in SC and IC immunized mice, with the highest levels seen in IC 
      immunized mice. Analysis of whole brain lysates revealed a strong induction of 
      immunoglobulin in the brains of IC immunized mice that had virus neutralizing 
      activity. Histopathological examination of brain tissue revealed mild 
      encephalitis and disseminated viral antigen in control and SC immunized mice, but 
      rare in IC immunized mice. These results suggest that IC immunization induces a 
      preventive humoral immune response against intracerebrally inoculated rabies 
      virus. Induction of neutralizing antibody in cerebrospinal fluid represents a 
      putative therapeutic measure for the treatment of rabid animals and humans.
CI  - Copyright (c) 2010 Institut Pasteur. Published by Elsevier SAS. All rights 
      reserved.
FAU - Sunden, Yuji
AU  - Sunden Y
AD  - Laboratory of Comparative Pathology, Graduate School of Veterinary Medicine, 
      Hokkaido University, N18 W9, Kita-ku, Sapporo 060-0818, Japan. 
      sunden@vetmed.hokudai.ac.jp
FAU - Yano, Shiori
AU  - Yano S
FAU - Ishida, Sachiyo
AU  - Ishida S
FAU - Ochiai, Kenji
AU  - Ochiai K
FAU - Umemura, Takashi
AU  - Umemura T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100814
PL  - France
TA  - Microbes Infect
JT  - Microbes and infection
JID - 100883508
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Rabies Vaccines)
RN  - 0 (Vaccines, Inactivated)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/analysis/blood
MH  - Antibodies, Viral/analysis/blood
MH  - Brain/*immunology/pathology
MH  - Disease Models, Animal
MH  - Female
MH  - Histocytochemistry
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Microscopy
MH  - Rabies/immunology/pathology/*prevention & control
MH  - Rabies Vaccines/*administration & dosage/*immunology
MH  - Rabies virus/*immunology
MH  - Vaccination/*methods
MH  - Vaccines, Inactivated/administration & dosage/immunology
PMC - PMC7110474
EDAT- 2010/08/18 06:00
MHDA- 2011/03/11 06:00
PMCR- 2010/08/14
CRDT- 2010/08/18 06:00
PHST- 2010/02/06 00:00 [received]
PHST- 2010/06/20 00:00 [revised]
PHST- 2010/08/04 00:00 [accepted]
PHST- 2010/08/18 06:00 [entrez]
PHST- 2010/08/18 06:00 [pubmed]
PHST- 2011/03/11 06:00 [medline]
PHST- 2010/08/14 00:00 [pmc-release]
AID - S1286-4579(10)00193-0 [pii]
AID - 10.1016/j.micinf.2010.08.002 [doi]
PST - ppublish
SO  - Microbes Infect. 2010 Dec;12(14-15):1163-9. doi: 10.1016/j.micinf.2010.08.002. 
      Epub 2010 Aug 14.