PMID- 20713177
OWN - NLM
STAT- MEDLINE
DCOM- 20110218
LR  - 20131121
IS  - 1532-0456 (Print)
IS  - 1532-0456 (Linking)
VI  - 153
IP  - 1
DP  - 2011 Jan
TI  - The influence of nutritional conditions on metal uptake by the mixotrophic dual 
      symbiosis harboring vent mussel Bathymodiolus azoricus.
PG  - 40-52
LID - 10.1016/j.cbpc.2010.08.004 [doi]
AB  - The vent mussel Bathymodiolus azoricus, host thioautotrophic and methanotrophic 
      bacteria, in their gills and complementary, is able to digest suspended organic 
      matter. But the involvement of nutritional status in metal uptake and storage 
      remains unclear. The influence of B. azoricus physiological condition on its 
      response to the exposure of a mixture of metals in solution is addressed. Mussels 
      from the Menez Gwen field were exposed to 50 mugL(-1) Cd, plus 25 mugL(-1) Cu and 
      100 mugL(-1) Zn for 24 days. Four conditions were tested: (i) mussels harboring 
      both bacteria but not feed, (ii) harboring only methanotrophic bacteria, (iii) 
      without bacteria but fed during exposure and (iv) without bacteria during 
      starvation. Unexposed mussels under the same conditions were used as controls. 
      Eventual seasonal variations were assessed. Metal levels were quantified in 
      subcellular fractions in gills and digestive gland. Metallothionein levels and 
      condition indices were also quantified. Gill sections were used for fluorescence 
      in situ hybridization (FISH) to assess the temporal distribution of symbiotic 
      associations. Starvation damages metal homeostasis mechanisms and increase the 
      intracellular Zn and MT levels function. There is a clear metallic competition 
      for soluble and insoluble intracellular ligands at each condition. Seasonal 
      variations were observed at metal uptake and storage.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Martins, Ines
AU  - Martins I
AD  - IMAR, DOP-Department of Oceanography and Fisheries, University of the Azores, 
      9901-862 Horta, Portugal. imartins@uac.pt
FAU - Bettencourt, Raul
AU  - Bettencourt R
FAU - Colaco, Ana
AU  - Colaco A
FAU - Sarradin, Pierre-Marie
AU  - Sarradin PM
FAU - Santos, Ricardo Serrao
AU  - Santos RS
FAU - Cosson, Richard
AU  - Cosson R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100813
PL  - United States
TA  - Comp Biochem Physiol C Toxicol Pharmacol
JT  - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
JID - 100959500
RN  - 0 (Metals)
RN  - 0 (Water Pollutants, Chemical)
RN  - 00BH33GNGH (Cadmium)
RN  - 789U1901C5 (Copper)
SB  - IM
MH  - Animals
MH  - Bacteria/drug effects
MH  - Cadmium/metabolism/toxicity
MH  - Copper/metabolism/toxicity
MH  - Digestive System/drug effects/metabolism/microbiology
MH  - Gills/drug effects/metabolism/microbiology
MH  - Metals/*metabolism/toxicity
MH  - Mytilidae/drug effects/*metabolism
MH  - Seawater/chemistry
MH  - Symbiosis/drug effects
MH  - Water Pollutants, Chemical/*metabolism/toxicity
EDAT- 2010/08/18 06:00
MHDA- 2011/02/22 06:00
CRDT- 2010/08/18 06:00
PHST- 2010/05/21 00:00 [received]
PHST- 2010/08/04 00:00 [revised]
PHST- 2010/08/05 00:00 [accepted]
PHST- 2010/08/18 06:00 [entrez]
PHST- 2010/08/18 06:00 [pubmed]
PHST- 2011/02/22 06:00 [medline]
AID - S1532-0456(10)00144-4 [pii]
AID - 10.1016/j.cbpc.2010.08.004 [doi]
PST - ppublish
SO  - Comp Biochem Physiol C Toxicol Pharmacol. 2011 Jan;153(1):40-52. doi: 
      10.1016/j.cbpc.2010.08.004. Epub 2010 Aug 13.