PMID- 20713312
OWN - NLM
STAT- MEDLINE
DCOM- 20120208
LR  - 20230228
IS  - 1532-2971 (Electronic)
IS  - 1090-0233 (Print)
IS  - 1090-0233 (Linking)
VI  - 189
IP  - 3
DP  - 2011 Sep
TI  - Immunisation of pigs with a major envelope protein sub-unit vaccine against 
      porcine reproductive and respiratory syndrome virus (PRRSV) results in enhanced 
      clinical disease following experimental challenge.
PG  - 323-9
LID - 10.1016/j.tvjl.2010.07.010 [doi]
AB  - Disease exacerbation was observed in pigs challenged with virulent porcine 
      reproductive and respiratory syndrome virus (PRRSV) following immunisation with a 
      recombinant GP5 sub-unit PRRSV vaccine (rGP5) produced in E. coli. Eighteen 
      animals were divided into three experimental groups: group A were immunised twice 
      IM with rGP5, 21 days apart; group B acted as positive controls (challenged but 
      not immunised); and group C were negative controls. Pigs in groups A and B were 
      challenged 21 days after the second immunisation of the group A animals. 
      Following challenge, three pigs given rGP5 exhibited more severe clinical signs 
      than the positive controls, including respiratory distress and progressive 
      weight-loss. Although not statistically significant, the more severe disease 
      exhibited by group A animals may suggest previous immunisation as a contributory 
      factor. The mechanisms of these findings remain unclear and no association could 
      be established between the severity of disease, non-neutralising antibody 
      concentrations and tissue viral loads.
CI  - Copyright (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Prieto, Cinta
AU  - Prieto C
AD  - Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense 
      de Madrid, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain.
FAU - Martinez-Lobo, Francisco Javier
AU  - Martinez-Lobo FJ
FAU - Diez-Fuertes, Francisco
AU  - Diez-Fuertes F
FAU - Aguilar-Calvo, Patricia
AU  - Aguilar-Calvo P
FAU - Simarro, Isabel
AU  - Simarro I
FAU - Castro, Jose Maria
AU  - Castro JM
LA  - eng
SI  - GENBANK/DQ345729
SI  - GENBANK/DQ345733
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100814
PL  - England
TA  - Vet J
JT  - Veterinary journal (London, England : 1997)
JID - 9706281
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Gene Products, env)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/blood
MH  - Base Sequence
MH  - Escherichia coli/immunology
MH  - Gene Products, env/*genetics/*immunology
MH  - Molecular Sequence Data
MH  - Porcine Reproductive and Respiratory 
      Syndrome/immunology/physiopathology/*prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*immunology/pathogenicity
MH  - Sequence Analysis, Protein
MH  - Sus scrofa
MH  - Swine
MH  - Swine Diseases/physiopathology/*prevention & control/virology
MH  - Vaccination/veterinary
PMC - PMC7172774
EDAT- 2010/08/18 06:00
MHDA- 2012/02/09 06:00
PMCR- 2010/08/14
CRDT- 2010/08/18 06:00
PHST- 2009/12/22 00:00 [received]
PHST- 2010/07/06 00:00 [revised]
PHST- 2010/07/12 00:00 [accepted]
PHST- 2010/08/18 06:00 [entrez]
PHST- 2010/08/18 06:00 [pubmed]
PHST- 2012/02/09 06:00 [medline]
PHST- 2010/08/14 00:00 [pmc-release]
AID - S1090-0233(10)00250-9 [pii]
AID - 10.1016/j.tvjl.2010.07.010 [doi]
PST - ppublish
SO  - Vet J. 2011 Sep;189(3):323-9. doi: 10.1016/j.tvjl.2010.07.010. Epub 2010 Aug 14.