PMID- 20716918
OWN - NLM
STAT- MEDLINE
DCOM- 20110112
LR  - 20211020
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2
IP  - 5
DP  - 2009 Nov-Dec
TI  - The fat-1 transgene in mice increases antioxidant potential, reduces 
      pro-inflammatory cytokine levels, and enhances PPAR-gamma and SIRT-1 expression 
      on a calorie restricted diet.
PG  - 307-16
LID - 10.4161/oxim.2.5.9579 [doi]
AB  - Both n-3 fatty acids (FA) and calorie-restriction (CR) are known to exert 
      anti-inflammatory and anti-oxidative effects in animals and humans. In this 
      study, we investigated the synergistic anti-inflammatory and anti-oxidative 
      capacity of n-3 FA and CR using Fat-1 transgenic mice (Fat-1) that are capable of 
      converting n-6 FA to n-3 FA endogenously. Wild type (WT) and Fat-1 mice were 
      maintained on ad libitum (AL) or CR (40% less than AL) AIN-93 diet supplemented 
      with 10% corn oil (rich in n-6 FA) for 5 months. Significantly lower levels of 
      n-6/n-3 FA ratio were observed in serum, muscle and liver of Fat-1 mice fed AL or 
      CR as compared to that of WT mice fed AL or CR. Muscle catalase (CAT), super 
      oxide dismutase (SOD), glutathione peroxidase (GPX) activities, and liver CAT and 
      SOD activities were found higher in Fat-1 mice as compared to that of WT mice. 
      These activities were more pronounced in Fat-1/CR group as compared to other 
      groups. Serum pro-inflammatory markers, such as tumor necrosis factor 
      (TNF)-alpha, interleukin (IL)-1beta, and IL-6 were found lower in Fat-1 mice, as 
      compared to that of WT mice. This anti-inflammatory effect was also more 
      pronounced in Fat-1/CR group as compared to that of other groups. Furthermore, 
      significantly higher levels of peroxisome proliferator-activated receptor 
      (PPAR)-gamma and life prolonging gene, sirtuin (SIRT)-1 expression were found in 
      liver of Fat-1/CR mice, as compared to that of WT/CR mice. These data suggest 
      that n-3 FA along with moderate CR may prolong lifespan by attenuating 
      inflammation and oxidative stress.
FAU - Rahman, Mizanur
AU  - Rahman M
AD  - Department of Medicine, University of Texas Health Science Center at San Antonio, 
      San Antonio, Texas, USA. rahmanm@uthscsa.edu
FAU - Halade, Ganesh V
AU  - Halade GV
FAU - Bhattacharya, Arunabh
AU  - Bhattacharya A
FAU - Fernandes, Gabriel
AU  - Fernandes G
LA  - eng
GR  - R01 AG030161/AG/NIA NIH HHS/United States
GR  - R01 AT004259/AT/NCCIH NIH HHS/United States
GR  - AT005232-01/AT/NCCIH NIH HHS/United States
GR  - -AG030161/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - 0 (Antioxidants)
RN  - 0 (Cadherins)
RN  - 0 (Cytokines)
RN  - 0 (Fatty Acids, Omega-3)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (PPAR gamma)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (fat1 protein, mouse)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 3.5.1.- (Sirtuin 1)
SB  - IM
MH  - Animals
MH  - Antioxidants/*metabolism
MH  - Cadherins/*genetics/metabolism
MH  - *Caloric Restriction
MH  - Catalase/metabolism
MH  - Cytokines/*metabolism
MH  - Fatty Acids, Omega-3/pharmacology
MH  - Glutathione Peroxidase/metabolism
MH  - Interleukin-1beta/blood/metabolism
MH  - Interleukin-6/blood/metabolism
MH  - Liver/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - PPAR gamma/*metabolism
MH  - Sirtuin 1/*metabolism
MH  - Superoxide Dismutase/metabolism
MH  - Tumor Necrosis Factor-alpha/blood/metabolism
PMC - PMC2835919
EDAT- 2010/08/19 06:00
MHDA- 2011/01/13 06:00
CRDT- 2010/08/19 06:00
PHST- 2010/08/19 06:00 [entrez]
PHST- 2010/08/19 06:00 [pubmed]
PHST- 2011/01/13 06:00 [medline]
AID - 9579 [pii]
AID - 1942-0900-2-5-7 [pii]
AID - 10.4161/oxim.2.5.9579 [doi]
PST - ppublish
SO  - Oxid Med Cell Longev. 2009 Nov-Dec;2(5):307-16. doi: 10.4161/oxim.2.5.9579.